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VECTASTAIN ABC-AmP Kit, for Mouse IgG, Chromogenic Western Blot Detection

Catalog Number: AK-6402

Availability: In stock

Unit Size: 1 kit

Western blot visualized by using the VECTASTAIN® ABC-AmP™ kits. Serial dilutions (1:2) of maltose binding protein (MBP) were resolved by electrophoresis on a 12% PAGE reducing gel, transferred onto nitrocellulose membrane and detected with biotinylated an Zoom



    • Amplified ABC alkaline phosphatase reagent for protein blot detection
    • Uses biotin/streptavidin technology
    • Contains highly sensitive chromogenic substrate, BCIP/NBT (indigo color)
    • Sufficient reagents to develop twenty 100 cm2 blots

    Kit Contents:

    • 250 ml 10x Casein Solution
    • 0.25 ml Biotinylated Anti-Mouse IgG
    • 0.5 ml Reagent A and 0.5 ml Reagent B for VECTASTAIN® ABC-AmP Reagent
    • BCIP/NBT Substrate Kit (produces 200 ml of substrate solution)

    Additional Information

    Catalog Number AK-6402
    Unit Size 1 kit
    CE Marked Yes
    Country of Manufacture United States
    Applications Blotting Applications, Elispot
    Technology ABC (Streptavidin/Biotin) Systems
    Detection Enzyme(s) Alkaline Phosphatase
    Target Species Mouse
    Format Concentrate
Additional Info


    Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.

    The VECTASTAIN® ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent/ chemifluorescent or a chromogenic substrate. Used with the DuoLuX™ Chemiluminescent/Chemifluorescent Substrate, the VECTASTAIN® ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.

    The sensitivity of VECTASTAIN® ABC-AmP Reagent combined with the DuoLux substrate allows for the detection of as little as 1 pg of target protein.

    Each VECTASTAIN® ABC-AmP kit provides sufficient reagents to develop approximately twenty 100 cm2 blots.

    Kit Contents:

    • VECTASTAIN® ABC-AmP reagent
    • 10x casein solution
    • biotinylated anti-mouse IgG secondary antibody
    • BCIP/NBT chromogenic substrate

    Reagents contained in the kits can also be purchased separately.


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