VECTASHIELD® PLUS Antifade Mounting Medium with DAPI

H-2000
SKU Unit Size Price Qty
H-2000-2 2 ml
$48.00
H-2000-10 10 ml
$164.00

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Description

VECTASHIELD PLUS Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. This medium inhibits rapid photobleaching of fluorescent proteins and fluorescent dyes. 

Features:

  • No inherent toning or background
  • Superior signal retention across the spectrum from blue to far red
  • Mounted sections can be viewed in 1 hour
  • DAPI (blue) counterstain premixed with medium
  • Available in two convenient sizes
  • Superior non-setting formulation

VECTASHIELD PLUS Mounting Medium with DAPI does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 2-8 °C, protected from light.

Specifications

More Information
Unit Size 2 ml, 10 ml
Applications Immunofluorescence, In situ hybridization
Mounting Aqueous (Non-Hardening)
Antifade Yes
Counterstain DAPI (blue fluorescence)

Documents

Technical Information

The refractive index for VECTASHIELD Mounting Medium is 1.45.

The VECTASHIELD PLUS Mounting Media are available with or without the counterstain DAPI (4', 6-diamidino-2-phenylindole). The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD PLUS Mounting Medium without DAPI. DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm, and is ideal as a nuclear or chromosomal counterstain. Additionally, VECTASHIELD PLUS Mounting Medium does not solidify, but rather, remains a liquid on the slide ‐ which can then be stored without sealing. If desired, coverslips can be sealed around the perimeter using nail polish or plastic sealant. Mounted slides should be stored at 2-8 °C and protected from light.

VECTASHIELD Mounting Medium Antifade Comparison

Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections that have been immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is then measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer's published results.

 

VECTASHIELD PLUS References:

1. Mahmud, F. et al. (2020) Orally available tubulin inhibitor VERU-111 enhances antitumor efficacy in paclitaxel-resistant lung cancer. Cancer Letters (https://www.sciencedirect.com/science/article/abs/pii/S0304383520304663)

VECTASHIELD Antifade signal retention after 3 minutes exposure
VECTASHIELD Products chart / table
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