Introducing VECTASHIELD® PLUS Antifade Mounting Media

VECTASHIELD PLUS is the second generation of the best in class antifade mounting media—VECTASHIELD. This unique, stable formula for preserving fluorescence inhibits rapid photobleaching of fluorescent proteins and fluorescent dyes. Our new non-setting formulation has been engineered to provide consistent, reliable signal retention of all commonly used fluorophores (from the visible to the far red) and eliminate blue background. VECTASHIELD PLUS enables you to see and do more with each experiment.

Human colon stained with Vectashield Plus — Human Colon: Rabbit Anti-Cytokeratin (AE1/AE3) and Mouse Anti-Desmin detected simultaneously with VectaFluor™ Duet Double Labeling Kit; Vasculature detected using DyLight® 649 UEA I Lectin (purple). Mounted in VECTASHIELD PLUS Antifade Mounting Medium (H-1900).

Human colon stained with Vectashield plus with DAPI — Human Colon: Rabbit Anti-Cytokeratin (AE1/AE3) and Mouse Anti-Desmin detected simultaneously with VectaFluor Duet Immunofluorescence Double Labeling Kit; Dylight 488 Anti-Mouse (green) / Dylight 594 Anti-Rabbit (red). Mounted in VECTASHIELD PLUS Antifade Mounting Medium with DAPI (nuclei, blue, H-2000).

Why VECTASHIELD PLUS Mounting Media?

No inherent toning or background
Superior signal retention from the visible to the far red spectrum
Mounted sections can be viewed in 1 hour
Available with or without DAPI counterstain
Can be purchased in 2 convenient sizes - 2ml or 10 ml
Compatible with commonly used fluorophores

Plus the same features you have come to expect of the VECTASHIELD brand:

Ideal refractive index
Easy to use
Ready to use, requires no warming
No sealing of coverslips is required (curing formulations)

Easy Application and Use of VECTASHIELD PLUS

Excellent Antifade Properties Across the Spectrum, with Commonly Used Fluorophores

VECTASHIELD PLUS Mounting Media provides excellent protection against fading across the visible spectrum, even under far-red wavelengths. VECTASHIELD PLUS is also compatible with most commercially available fluorophores.

Vectashield plus shows excellent antifade properties across the spectrum

Retention of signal strength after mounting +++ Excellent ++++ Superior

Key features of VECTASHIELD PLUS

1) No background toning

No inherent background provides sharper contrast with surrounding tissue elements for superior signal interpretation and imaging.

Vectashield Plus with DAPI image staining shows no background toning — VECTASHIELD PLUS with DAPI (H-2000).
*Note black background and excellent contrast*

Competitor to Vectashield Plus shows a hazy background and poor contrast — Leading non-setting antifade mounting medium with DAPI.
*Note hazy background and poor contrast*.

2) View sections one-hour after mounting

Serial sections stained for cytokeratin using CY 5 and imaged one-hour after mounting following a 3-minute exposure under the fluorescent microscope.

Vectashield Plus shows retention of strong specific signal, 1 hour after mounting — Mounted with VECTASHIELD PLUS (H-1900).
*Note retention of strong specific signal.*

Vectashield Plus competitor shows poor signal after 1 hour — Mounted with non-setting antifade media from Company N.
*Note almost complete absence of specific signal.*

Vectashield Plus shows high percentage of signal retention after 1 hour — Percentage of Signal Retention After 3-Minute Exposure 1 hour post-mounting.

3) Superior signal retention to enhance your assays

VECTASHIELD PLUS is suitable for use with traditional and contemporary fluorophores across the visible and far-red spectrum. The graph to the right indicates fluorescence signal retention on tissue sections mounted with VECTASHIELD PLUS and imaged 1 hour after mounting following a 3-minute exposure under a fluorescent microscope.

Vectashield Plus shows strong signal retention with 3 different fluorophores after 1 hour post-mounting

4) Choices to suit your needs

VECTASHIELD PLUS is offered with or without DAPI counterstain and is available for purchase in convenient 2 ml and 10 ml unit-sized formats.

Vectashield Plus H-1900 colon stained with cytokeratin red and Ki67 green — VECTASHIELD PLUS (H-1900).
Colon section stained for cytokeratin (red) and Ki67 (green).

Vectashield Plus with DAPI H-2000 colon stained with cytokeratin green — VECTASHIELD PLUS with DAPI (H-2000).
Colon section stained for cytokeratin (green). Nuclear DAPI (blue) staining..

How does VECTASHIELD PLUS compare to the competition

Explore our entire portfolio of VECTASHIELD Antifade Mounting Media Products

Excellent antifade properties of VECTASHIELD® PLUS™ Mounting Media

Ordering Information

Product	Unit Size	No Counterstain	With DAPI Counterstain	With PI Counterstain	TRITC-Phalloidin
VECTASHIELD PLUS Antifade Mounting Medium	2ml, 10ml	H-1900	H-2000
VECTASHIELD Vibrance Antifade Mounting Medium (hardening)	2ml, 10ml	H-1700	H-1800
VECTASHIELD Antifade Mounting Medium (non-hardening)	10ml	H-1000	H-1200	H-1300
VECTASHIELD Hardset™ Antifade Mounting Medium (hardening)	10ml	H-1400	H-1500		H-1600