VECTASHIELD® PLUS Antifade Mounting Medium

H-1900
SKU Unit Size Price Qty
H-1900-2 2 ml
$46.00
H-1900-10 10 ml
$152.00

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Description

VECTASHIELD PLUS Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. This medium inhibits rapid photobleaching of fluorescent proteins and fluorescent dyes.

Features:

  • No inherent toning or background
  • Superior signal retention across the spectrum from blue to far red
  • Mounted sections can be viewed in 1 hour
  • Available in two convenient sizes
  • Superior non-setting formulation

Specifications

More Information
Unit Size 2 ml, 10 ml
Applications Immunofluorescence, In situ hybridization
Recommended Storage 2-8 ºC
Mounting Aqueous (Non-Hardening)
Antifade Yes
Counterstain None

Documents

Product FAQs

What are the advantages of VECTASHIELD PLUS over regular VECTASHIELD (H-1000 & H-1200)?​

VECTASHIELD PLUS provides several advantages over the regular VECTASHIELD (H-1000 & H-1200). In particular, VECTASHIELD PLUS media have significantly lower inherent background​ toning and provide superior signal retention particularly in the far-red spectrum (e.g. Cy5).

Does the coverslip need to be sealed with nail polish or similar compound​ after mounting?

Similar to other non-setting media, we recommend you to seal the coverslip perimeter if the intention is to store the slides for a period of time.

Does VECTASHIELD PLUS perform equivalently or better with Alexa Fluor dyes​ compared with the regular VECTASHIELD (H-1000 & H-1200)? ​

Yes, the antifade mounting media are at least equivalent in signal retention. We have not seen any reduction in performance with any fluorophores evaluated, and with some dyes (i.e. Alexa Fluor 647 and Dylight 649) there are advantages. 

Is VECTASHIELD PLUS suitable for super resolution microscopy (SRM)?​

We do know that regular VECTASHIELD antifade mounting media (H-1000 & H-1200) are suitable for SRM.​ However, the formulation for VECTASHIELD PLUS is different from that used for regular VECTASHIELD. Indeed one component of H-1000 and​ H-1200 that may have been a contributing factor to the underlying blue​ tone and that appears to play a key role in optimal signal for SRM, has​ been removed from VECTASHIELD PLUS. Therefore, until we have data to the ​contrary VECTASHIELD PLUS would not be recommended for SRM.

Technical Information

The refractive index for VECTASHIELD Mounting Medium is 1.45.

VECTASHIELD PLUS Mounting Medium does not solidify, but rather, remains a liquid on the slide ‐ which can then be stored without sealing. If desired, coverslips can be sealed around the perimeter using nail polish or plastic sealant. Mounted slides should be stored at 2-8 °C and protected from light.

VECTASHIELD Mounting Medium Antifade Comparison

Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections that have been immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is then measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer's published results.

 

VECTASHIELD PLUS References:

1. González-Rodríguez, P. et al. (2020) The DNA methyltransferase DNMT3A contributes to autophagy long-term memory. Autophagy. (https://www.tandfonline.com/doi/full/10.1080/15548627.2020.1816664)

VECTASHIELD Fluorophore Signal Table Comparison
VECTASHIELD Antifade signal retention after 3 minutes exposure
VECTASHIELD Products chart / table
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