VECTASHIELD® Hardset™ Antifade Mounting Medium

Cat. No: H-1400
Price: $160.00
Unit Size: 10 ml


VECTASHIELD Hardset Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD Hardset Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. VECTASHIELD Hardset Mounting Medium hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.


  • Inhibits photobleaching of dyes and fluorescent proteins
  • Ideal refractive index
  • Ready to use
  • No warming necessary
  • Offered with nuclear or cytoskeletal counterstains
  • Available in hardening formulation
  • Can be stored without sealing for long term analysis
  • Easy to use
Cat. No. H-1400-10
Unit Size 10 ml
Country of Manufacture United States
Applications Immunofluorescence, In situ hybridization, Cellular Imaging
Mounting Aqueous (Hardening)
Antifade Yes
Counterstain None

More Information

VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (under the "Protocol/Data Sheet/SDS" tab) to determine if VECTASHIELD will be compatible in your system.

R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.

Refractive index is 1.46.

VECTASHIELD Mounting Medium Antifade Comparison

Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer s published results.

VECTASHIELD Antifade Comparison
VECTASHIELD HardSet Antifade properties

Safety Information

Warning Icon WARNING: