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VECTASHIELD HardSet Antifade Mounting Medium with Phalloidin

Catalog Number: H-1600

Availability: In stock

$145.00
Unit Size: 10 ml

Mouse embryonic fibroblast stained with mouse anti-tubulin followed by fluorescein horse anti-mouse IgG and mounted in VECTASHIELD HardSet Mounting Medium with DAPI and TRITC-Phalloidin. Zoom

Overview

    Features:

    • Inhibits photobleaching of fluorescent dyes and fluorescent proteins
    • Ideal refractive index (1.46)
    • Ready-to-use - from the refrigerator to the benchtop
    • No warming necessary
    • Can be stored without sealing for long term analysis
    • Hardening formulation
    • TRITC-Phalloidin counterstain

    Additional Information

    Catalog Number H-1600
    Unit Size 10 ml
    Country of Manufacture United States
    Applications Immunofluorescence, In situ hybridization, Cellular Imaging
    Mounting Aqueous (Hardening)
    Antifade Yes
    Counterstain Phalloidin Rhodamine (red fluorescence)
    Cellular Stain Cytoskeleton
Additional Info

    Details

    VECTASHIELD® Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD® Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.

    Features:

    • Inhibits photobleaching of dyes and fluorescent proteins
    • Ideal refractive index
    • Ready to use
    • No warming necessary
    • Offered with nuclear or cytoskeletal counterstains
    • Available in non-hardening or hardening formulations
    • Can be stored without sealing for long term analysis
    • Easy to use

    The original VECTASHIELD® Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 °C, protected from light.

    VECTASHIELD® HardSet™ Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved. 

    VECTASHIELD® HardSet™ Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (under the "Protocol/Data Sheet/MSDS" tab) to determine if VECTASHIELD® will be compatible in your system.

    This special formulation of VECTASHIELD® HardSet™ Mounting Medium contains TRITC-phalloidin. Phalloidin is a bicyclic heptapeptide that specifically binds at the interface between the F-actin subunits. Fluorescent derivatives of phalloidin are used to stain actin filaments. TRITC (tetramethylrhodamine) is excited at 544 nm and emits at 572 nm, producing an orange-red fluorescence.

    R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.

    The refractive index for VECTASHIELD® HardSet™ Mounting Medium is 1.46.

    NOTE: This product has a six month expiration date.

    VECTASHIELD® Mounting Medium Antifade Comparison 

    Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD® mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer’s published results.

    VECTASHIELD Antifade Comparison

     

    VECTASHIELD HardSet antifade properties

     

    VECTASHIELD Products

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Protocols/Data Sheet/SDS