|Unit Size||10 ml|
|Country of Manufacture||United States|
|Applications||Immunofluorescence, In situ hybridization, Cellular Imaging|
|Counterstain||Phalloidin Rhodamine (red fluorescence)|
VECTASHIELD® Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD® Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.
The original VECTASHIELD® Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 °C, protected from light.
VECTASHIELD® HardSet™ Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.
VECTASHIELD® HardSet™ Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (under the "Protocol/Data Sheet/MSDS" tab) to determine if VECTASHIELD® will be compatible in your system.
This special formulation of VECTASHIELD® HardSet™ Mounting Medium contains TRITC-phalloidin. Phalloidin is a bicyclic heptapeptide that specifically binds at the interface between the F-actin subunits. Fluorescent derivatives of phalloidin are used to stain actin filaments. TRITC (tetramethylrhodamine) is excited at 544 nm and emits at 572 nm, producing an orange-red fluorescence.
R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.
The refractive index for VECTASHIELD® HardSet™ Mounting Medium is 1.46.
NOTE: This product has a six month expiration date.
Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD® mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer’s published results.