VECTASHIELD® Hardset™ Antifade Mounting Medium with DAPI

Cat. No: H-1500
Price: $170.00
Unit Size: 10 ml


VECTASHIELD Hardset Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. VECTASHIELD Hardset Mounting Medium with DAPI prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. VECTASHIELD Hardset Mounting Medium with DAPI hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved. 


  • Inhibits photobleaching of fluorescent dyes and fluorescent proteins
  • Ideal refractive index (1.46)
  • Ready-to-use - from the refrigerator to the benchtop
  • No warming necessary
  • Can be stored without sealing for long term analysis
  • Hardening formulation
  • DAPI counterstain
Cat. No. H-1500-10
Unit Size 10 ml
Country of Manufacture United States
Applications Immunofluorescence, In situ hybridization, Cellular Imaging
Mounting Aqueous (Hardening)
Antifade Yes
Counterstain DAPI (blue fluorescence)
Cellular Stain Nucleus

More Information

VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (under the "Protocol/Data Sheet/SDS" tab) to determine if VECTASHIELD will be compatible in your system.

Both the VECTASHIELD Hardset and the original VECTASHIELD Mounting Media are available with or without the counterstain DAPI (4', 6-diamidino-2-phenylindole). The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD Mounting Medium without DAPI.  DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.

R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.

The refractive index for VECTASHIELD Hardset Mounting Medium is 1.46.

NOTE: This product has a six month expiration date.

VECTASHIELD Mounting Medium Antifade Comparison

Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer s published results.

VECTASHIELD Anifade Comparison
VECTASHIELD HardSet Antifade Properties

Safety Information

Warning Icon WARNING: