VECTASHIELD Hardset Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. VECTASHIELD Hardset Mounting Medium with DAPI prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. VECTASHIELD Hardset Mounting Medium with DAPI hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.
|Unit Size||10 ml|
|Country of Manufacture||United States|
|Applications||Immunofluorescence, In situ hybridization, Cellular Imaging|
|Counterstain||DAPI (blue fluorescence)|
VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (under the "Protocol/Data Sheet/SDS" tab) to determine if VECTASHIELD will be compatible in your system.
Both the VECTASHIELD Hardset and the original VECTASHIELD Mounting Media are available with or without the counterstain DAPI (4', 6-diamidino-2-phenylindole). The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD Mounting Medium without DAPI. DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.
R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.
The refractive index for VECTASHIELD Hardset Mounting Medium is 1.46.
NOTE: This product has a six month expiration date.
Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer s published results.
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