VectaMount® Permanent Mounting Medium

H-5000-60
SKU Unit Size Price Qty
H-5000-60 60 ml
$128.00

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Description

VectaMount Mounting Medium (H-5000) is an optically clear and odorless formula for permanently preserving histochemical stains or precipitable enzyme substrates in tissue sections or cell preparations. VectaMount Mounting Medium contains no toluene or xylene.

Features:

  • Permanent, non-aqueous mounting 
  • Low hazard 
  • Compatible with horseradish peroxidase, alkaline phosphatase, and glucose oxidase substrates 
  • Refractive index: 1.49 when dry

Specifications

More Information
Unit Size 60 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended Usage Following dehydration and clearing of the tissue section or cell preparation on a slide, dispense a small amount of VectaMount Mounting Medium on or adjacent to the section using a pipette (plastic or glass) or glass rod. As the coverslip is applied, the medium will disperse over the entire coverslipped area. Allow the preparation to dry. This may take one to several hours, depending on the temperature, humidity, type of clearing agent used, and amount of mounting medium applied. Drying can be accelerated by incubating the slide in a 60 ºC to 75 ºC oven for 15 to 30 minutes. Allow the slide to cool before storage. VectaMount Mounting Medium dries clear with an ideal refractive index. Mounted slides can be viewed with high magnification oil immersion lenses.Also, when mounting preparations stained with the BCIP/NBT substrate, crystal formation that may occur when using other media is minimized. If necesary, the coverslip can be removed by incubating the slide in xylene or a d-limonene-based clearing agent.Removing the coverslip using a d-limonene-based product may require a longer incubation time. Keep container tightly sealed when not in use.Some cloudiness of the solution may be present but will have no adverse affect on the appearance of the mounted section.
Recommended Storage Room Temperature
Mounting Non-Aqueous (Permanent)
Antifade No

Documents

Product FAQs

In some IHC detection reagent instructions, the last step states “dehydrate, clear and mount”. What does this mean?

In a manual IHC application, these steps involve submersion of the slides into a graded ethanol series, immediately followed by submersion into a miscible solution (xylene or alternative), followed by placement of a coverslip over the preparation with a suitable mounting medium. These steps preserve the specimen for subsequent visualization, imaging and archiving. From our experience, we suggest moving the slides in a slide rack through a series of dishes, commencing dehydration at 95% ethanol (2 changes for 2 min ea.), then into 100% ethanol (2x 2 min ea.), then into clearing solution (3x 2 min ea.), followed by mounting (coverslipping).

Citations

Technical Information

VectaMount Mounting Medium has a viscosity which provides for easy application and uniform spreading over the tissue section. Mounted sections are clear with an ideal refractive index suitable for high resolution oil immersion microscopy. VectaMount™ Mounting Medium preserves the color and intensity of preparations stained with enzyme substrates such as DAB, TMB, and BCIP/NBT, as well as our proprietary substrates Vector® NovaRED™ , Vector VIP, Vector SG, ImmPACT® DAB, ImmPACT DAB EqV, ImmPACT NovaRED, ImmPACT VIP, ImmPACT SG, Vector Red, ImmPACT Vector Red, Vector Blue, and Vector Black. The crystal formation that frequently occurs with the alkaline phosphatase substrate BCIP/NBT using other permanent mounting media is essentially eliminated.

 

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