VectaFluor R.T.U. DyLight® 488 Anti-Mouse IgG

Cat. No: DI-2788
Price: $87.00
Unit Size: 1 kit

Overview

Features:

  • Ready-to-use format offers maximum convenience for fluorescence staining of cells and tissues
  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
  • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L) 
  • Optimally labeled with DyLight® 488 to provide the brightest label for fluorescence microscopy 
  • Excitation: 493 nm 
  • Emission: 518 nm 
  • Color: Green

Kit Contents:

  • 15 ml pre-diluted, stabilized secondary antibody solution 
  • 15 ml 2.5% normal horse serum for blocking
Cat. No. DI-2788-15
Unit Size 1 kit
Country of Manufacture United States
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Target Species Mouse
Detection Target N/A
Conjugate DyLight 488
Color of Fluorescence Green
Host Species Horse
Format Ready-to-Use

More Information

VectaFluor™ Ready-to-Use (R.T.U.) DyLight® dye-labeled Secondary Antibodies offer maximum convenience for fluorescence staining of cells and tissues. Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight® dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. DyLight® dyes offer several advantages such as brighter fluorescence, greater photostability, and pH independence.

VectaFluor™ R.T.U. Antibody Reagents are supplied as 15 ml pre-diluted, stabilized, ready-to-use solutions along with 15 ml ready-to-use 2.5% normal horse serum for blocking.

  • Excitation maximum is at 493 nm
  • Emission maximum is at 518 nm
  • Color:  Green

DyLight® fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight® dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight® dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight® dyes can be applied as single labels or in combination with other DyLight® dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The Dylight® dyes currently offered are DyLight® 488 (green), DyLight® 549 (orange), DyLight® 594 (red), and DyLight® 649 (far red).

 

DyLight Spectra

DyLight Table

Protocols/Data Sheets/SDS

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