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VectaFluor Duet Immunofluorescence Double Labeling Kit, DyLight 488 Anti-Rabbit (green)/DyLight 594 Anti-Mouse (red)

Catalog Number: DK-8818

Availability: In stock

$120.00
Unit Size: 1 kit

Prostate:  Rabbit Anti-PSA MAb and Mouse Anti-Smooth Muscle Actin detected simultaneously with VectaFluor Duet Immunofluorescence Double Labeling Kit, DyLight 488 Anti-Rabbit (green)/DyLight 594 Anti-Mouse (red).  Mounted in VECTASHIELD HardSet Mounting M Zoom

Overview

    Features:

    • Ready-to-use format offers maximum convenience for fluorescence staining of cells and tissues
    • Robust cocktail formulation of DyLight anti-mouse IgG/anti-rabbit IgG secondary antibodies
    • Will detect both mouse and rabbit primary antibodies with green and red DyLight fluorescent dyes in a single step
    • Affinity-purified, ultrapure, high affinity antibodies
    • Secondary detection reagent optimally labeled with DyLight® dyes to provide the brightest label for fluorescence microscopy 

    Kit Contents:

    • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking 
    • 15 ml VectaFluor™ Duet Reagent [DyLight® 488 Anti-Rabbit IgG and DyLight®594 Anti-Mouse IgG cocktail (rabbit-green, mouse-red)] made in horse, ready-to-use in 5% NHS. 

    DyLight® 488:

    • Excitation maximum:   493 nm
    • Emission maximum:  518 nm
    • Color:  Green

    DyLight®594:

    • Excitation maximum:   592 nm
    • Emission maximum:  617 nm
    • Color:  Red

    Product Citations:

    Additional Information

    Catalog Number DK-8818
    Unit Size 1 kit
    Country of Manufacture United States
    Applications Immunofluorescence
    Target Species Universal (Mouse/Rabbit)
    Conjugate DyLight 488/DyLight 594
    Color of Fluorescence Kit (Green, Red)
    Host Species Horse
    Format Ready-to-Use
Additional Info

    Details

    VectaFluor™ Duet Kit offers maximum convenience to achieve immunofluorescence double labeling in one step. This kit includes a ready-to-use detection reagent that will detect mouse and rabbit primary antibodies with green and red DyLight® fluorescent dyes in a single step.  Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight®dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. VectaFluor™ Duet is compatible with fluorescence staining of cells and tissues.

    Kit Contents:

    • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking 
    • 15 ml VectaFluor™ Duet Reagent [DyLight® 488 Anti-Rabbit IgG and DyLight® 594 Anti-Mouse IgG cocktail (rabbit-green, mouse-red)] made in horse, ready-to-use in 5% NHS. 

    DyLight® 488:

    • Excitation maximum:   493 nm
    • Emission maximum:  518 nm
    • Color:  Green

    DyLight® 594:

    • Excitation maximum:   592 nm
    • Emission maximum:  617 nm
    • Color:  Red

    DyLight® fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

    DyLight® dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight® dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight® dyes can be applied as single labels or in combination with other DyLight® dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The Dylight® dyes currently offered are DyLight® 488 (green), DyLight® 549 (orange), DyLight® 594 (red), and DyLight® 649 (far red).

     

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Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    Kit Instructions SDS

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