VectaCell™ Trolox Antifade Reagent for Live Cell Imaging

CB-1000
SKU Unit Size Price Qty
CB-1000-2 2 ml
$100.00

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Description

VectaCell™ Trolox is an antifading additive for live cell imaging. It is a stock solution of the antioxidant Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) in filtered ethanol. Trolox is a water-soluble and cell-permeable analog of vitamin E, that efficiently prevents formation of different reactive oxygen species, such as singlet oxygen (1O2), superoxide anion (O2-) or hydrogen peroxide (H2O2).

Specifications

More Information
Unit Size 2 ml
Applications Immunofluorescence, Cellular Imaging
Concentration 100 mM (in ethanol)
Recommended Usage Instructions for use: 1. Prepare Acridine Orange labeling solution by diluting the stock solution 1:1000 with modified PBS (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM CaCl2, 0.5 mM MgCl2, pH 7.4). 2. Remove the culture media from cells. 3. Rinse cells three times with PBS. 4. Incubate cells in Acridine Orange labeling solution at 37°C for 15 minutes. 5. Remove labeling solution and rinse cells three times with PBS . 6. Cells grown on coverslips should be mounted onto microscope slides using PBS. 7. Image cells under the fluorescence microscope.
Recommended Storage 2-8 °C
Microscopy Fluorescence
Antifade Yes

Documents

Technical Information

VectaCell™ Trolox contains both Trolox and its oxidized form Trolox-quinone. This redox system reduces photo-bleaching and blinking during live cell imaging. 1, 2 Trolox has been shown to have a cytoprotective effect and low cytotoxicity for different cell lines.3

Photo-excitation of a fluorophore generates reactive oxygen species that can lead to photo-bleaching as well as oxidative damage in cells. Although reduction of the light intensity usually decreases fading, it also reduces the sensitivity of the assay.

Trolox has been used to reduce fluorophore bleaching1 as well as the fluorophore blinking2 during the observation of fluorescent labels. Although the mechanism is not completely understood it is attributed to the combination of its oxygen-scavenger capacity that prevents oxidative bleaching and the complementary reduction reaction that helps prevent blinking. It is supplied as a convenient 100 mM stock solution


References:

1. Rasnik et al. (2006) Nat. Meth. 3:891-893

2. Cordes et al. (2009) J. Am. Chem. Soc. 131:5018-5019

3. Wu et al. (1990) Biochem. Cell Biol. 68: 1189-1194