VECTABOND® Reagent, Tissue Section Adhesion

SP-1800
SKU Unit Size Price Qty
SP-1800-7 7 ml
$125.00

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Description

The VECTABOND® Reagent for Tissue Section Adhesion is designed to significantly increase the adherence of both frozen and paraffin-embedded tissue sections and cell preparations to glass slides and coverslips. It chemically modifies the surface of the glass with positive charges to form a highly adherent surface.

Features:

  • Tissues remain attached even under extreme conditions such as high temperature antigen unmasking techniques and in situ hybridization
  • VECTABOND Reagent treated slides can be stored for long periods
  • 7 ml concentrate dilutes to 350 ml
  • Treats at least 500 standard slides

Specifications

More Information
Unit Size 7 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization
Recommended Usage VECTABOND™ REAGENT INSTRUCTIONS Note:Use acetone-compatible containers such as glass staining dishes for these reagents. 1. Slide Preparation: Place clean glass slides in metal slide racks.Use either good quality pre-cleaned slides or wash slides thoroughly in detergent and rinse in water.Immerse slides in acetone for 5 minutes. 2. Slide Treatment: Prepare VECTABOND™ Reagent treatment solution by adding the entire contents (7 ml) of the bottle to 350 ml of acetone and stirring well.Remove slides from acetone, tapping rack several times to drain and immediately place in VECTABOND™ Reagent solution for 5 minutes.Remove slides and drain.Eliminate excess reagent by gently dipping them several times over 30 seconds in deionized or distilled water (do not create bubbles), changing the water after every 5 racks.Remove rack of slides for drying.Gentle agitation or tapping of the rack before allowing to dry will decrease water droplets and their resulting spots.Air dry slides thoroughly at room temperature or at 37°C. VECTABOND™ Reagent solution should be prepared just prior to use.The 357 ml of solution fills a standard size horizontal staining dish and is sufficient to treat at least 500 slides. Slides can be used immediately after drying, or stored in a box at room temperature. 3. Section Adhesion: For paraffin embedded tissue, float sections onto VECTABOND™ Reagent treated slides in a 45°C water bath.Air dry sections thoroughly.Heat sections at 56°C for 30 minutes to affix sections well.Most tissue sections will bind well under these conditions.If there is a problem with section adhesion, heating the sections for up to 24 hours at 56°C or for 1 hour at 75°C will increase adhesion.For immunohistochemical applications, temperatures above 60°C are not recommended because loss of antigens may occur. Store slides in a box at room temperature.When needed, deparaffinize and process slides as usual. For frozen tissue, allow sections to dry on VECTABOND™ Reagent treated slides before fixation and storage.Process slides as usual.
Recommended Storage Room temperature. For best results, we recommend that the entire contents be used at once. If a partially used bottle is to be kept, store the sealed bottle at room temperature and protect from moisture.

Documents

Product FAQs

How long can I store VECTABOND® working solution once diluted in acetone?

VECTABOND Reagent is supplied as a 7 ml concentrate that is diluted in acetone immediately prior to use. Once diluted in acetone, as per the instructions for use, it is recommended that the working solution be used as soon as possible. Storage of this working solution is not practical beyond a few hours as the chemicals will start to break down and the solution will turn a yellowish color. Once this has happened, slide coating will not be optimal and the working solution should be discarded.

Citations

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