Unconjugated Phaseolus Vulgaris Erythroagglutinin (PHA-E)

Cat. No: L-1120
Price: $142.00
Unit Size: 5 mg

Overview

Phaseolus vulgaris agglutinin is the name ascribed to a family of lectins, each of which consists of four subunits. There are two different types of subunits. One appears to be involved primarily in red cell agglutination and has been designated the “E” subunit (for erythroagglutinin). The other type is involved in lymphocyte agglutination and mitogenic activity and has been termed the “L” subunit (for leucoagglutinin). These subunits combine to produce five isolectins. PHA-E possesses strong hemagglutinating activity but is a poor mitogen.
Cat. No. L-1120-5
Unit Size 5 mg
Country of Manufacture United States
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Conjugate Unconjugated
Sugar Specificity Galactose, Complex Structures

More Information

Phaseolus vulgaris agglutinin is the name ascribed to a family of lectins, each of which consists of four subunits. There are two different types of subunits. One appears to be involved primarily in red cell agglutination and has been designated the E subunit (for erythroagglutinin). The other type is involved in lymphocyte agglutination and mitogenic activity and has been termed the L subunit (for leucoagglutinin). These subunits combine to produce five isolectins.

One of these isolectins has four E subunits and is designated PHA-E. PHA-E possesses strong hemagglutinating activity but is a poor mitogen. PHA-L, with four L type subunits, does not agglutinate red cells but is a potent mitogen. The other three isolectins, designated E3L1, E2L2, and E1L3, have erythroagglutinating and mitogenic activities proportional to the number of respective E or L subunits. We have termed the mixture of the five isolectins PHA (E+L).

PHA-L has been found to be an excellent, specific marker for use in anterograde neuronal tracing.

Elution: 100 mM acetic acid

Protocols/Data Sheets/SDS