Unconjugated Hippeastrum Hybrid (Amaryllis) Lectin (HHL, AL)

L-1380
SKU Unit Size Price Qty
L-1380-5 5 mg
$168.00

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Description

Amaryllis lectin binds only α-mannose residues, unlike Con A, LCA and PSA that also bind α-glucosyl structures. Like NPL there appears to be an extended binding site for polymannose structures, not requiring mannose to be at the non-reducing terminus. HHL binds both (α-1,3) and (α-1,6) linked mannose structures, as well as some yeast galactomannans.

Specifications

More Information
Unit Size 5 mg
Applications Glycobiology
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended.For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Conjugate Unconjugated
Sugar Specificity Mannose

Documents

Technical Information

This lectin is composed of four subunits of identical size and is immunologically related to GNL and NPL. Amaryllis lectin binds only α-mannose residues, unlike Con A, LCA and PSA that also bind α-glucosyl structures. Like NPL there appears to be an extended binding site for polymannose structures, not requiring mannose to be at the non-reducing terminus. HHL binds both (α-1,3) and (α-1,6) linked mannose structures, as well as some yeast galactomannans.

Inhibiting Sugar: 100 mM α-methylmannoside