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VECTASHIELD Antifade Mounting Medium

Catalog Number: H-1000

Availability: In stock

Unit Size: 10 ml

3D STORM imaging of Alexa Fluor 647 stained microtubules in a VECTASHIELD Mounting Medium/TRIS-Glycerol mixture. (Image kindly supplied by Dr. Nicolas Olivier, King's College, London UK.) Zoom

Overview

    CE markedFeatures:

    • Inhibits photobleaching of fluorescent dyes and fluorescent proteins
    • Ideal refractive index (1.45)
    • Ready-to-use - from the refrigerator to the benchtop
    • No warming necessary
    • Can be stored without sealing for long term analysis
    • Non-hardening formulation
    • No counterstain

    Additional Information

    Catalog Number H-1000
    Unit Size 10 ml
    CE Marked Yes
    Country of Manufacture United States
    Applications Immunofluorescence, In situ hybridization, Cellular Imaging, Super-Resolution Microscopy
    Mounting Aqueous (Non-Hardening)
    Antifade Yes
    Counterstain None
Additional Info

    Details

    VECTASHIELD® Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD® Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.

    Features:

    • Inhibits photobleaching of dyes and fluorescent proteins
    • Ideal refractive index
    • Ready to use
    • Offered with nuclear or cytoskeletal counterstains
    • Available in non-hardening or hardening formulations
    • No warming necessary
    • Can be stored without sealing for long term analysis
    • Easy to use

    The original VECTASHIELD® Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 °C, protected from light.

    VECTASHIELD® HardSet™ Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved. 

    VECTASHIELD® Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (under the "Protocol/Data Sheet/SDS" tab) to determine if VECTASHIELD® will be compatible in your system.

    R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.

    The refractive index for VECTASHIELD® Mounting Medium is 1.45.

    VECTASHIELD® Mounting Medium in Super Resolution Microscopy

    The optimal medium for super-resolution imaging methods maximizes the lifetime and photo-switching characteristics of fluorophores. VECTASHIELD® Mounting Medium has been shown to be compatible with a number of fluorophores used in super resolution methods including STORM, STED, and 3D-SIMM imaging, such as Cy5 or Alexa fluor 647, resulting in greater convenience and reproducibility of the method. (Olivier, N. et al., 2013, Biomedical Optics Express, Vol. 4 No. 6, pp. 885-899).

    VECTASHIELD® Mounting Medium Antifade Comparison

    Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD® mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies.  Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer s published results.

    VECTASHIELD Comparison

    VECTASHIELD Antifade Properties
    VECTASHIELD Products
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Protocols/Data Sheet/SDS