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Anti-Streptavidin and Anti-Avidin Reagents

Amplification of fluorescent signal using biotinylated anti-streptavidin or biotinylated anti-avidinBiotinylated Anti-Streptavidin and Biotinylated Anti-Avidin antibodies provide an ideal method to further increase sensitivity. These antibodies bind to streptavidin or avidin, respectively, through both their antigen binding sites and also through the covalently attached biotin residues. After the first application of a fluorochrome-labeled streptavidin or avidin, the signal is amplified by incubation with Biotinylated Anti- Streptavidin or a Biotinylated Anti-Avidin antibody followed by a second incubation with fluorochrome-labeled streptavidin or avidin. This procedure results in the introduction of more fluorochromes at the target site.

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