To Order: (01733) 237999

Blocking Solutions for Immunofluorescence

Clean, reliable, reproducible results can be attained by using appropriate detection reagents, running informative negative controls, and choosing the right blocking solutions. Negative controls will help determine the factors that contribute most to background staining. An appropriate series of controls is outlined in Vector's Trouble Shooting Guide (available for free download or as a convenient laminated copy for the lab).

Blocking agents are chosen based on results from these negative control sections, and the appropriate ones can prevent background interference from different sources including endogenous enzyme activity, presence of Fc receptors, or interactions of detection reagents with tissue/cell proteins and other macromolecules. The chart below suggests the blocking reagents suitable to reduce background staining.

16 Showing

List  Grid 

16 Showing

List  Grid 

Didn't find what you were looking for? Submit a suggestion.