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VectaCell Acridine Orange for Live Cell Imaging of Organelles

Catalog Number: CB-2000

Availability: In stock

Unit Size: 2 ml

Acidic endosomes stained with VectaCell Acridine Orange in MCF-7 cells expressing GFP. Zoom

Overview

    Features:

    • A fluorescent dye used to stain acidic organelles (lysosomes, autosomes or yeast vacuoles) in live cells
    • At low pH (inside the organelles) - emits an orange fluorescence (peak at 590 nm)
    • Supplied as a convenient stock solution (makes 2 L total solution).

    Additional Information

    Catalog Number CB-2000
    Unit Size 2 ml
    Country of Manufacture United States
    Applications Immunofluorescence, Cellular Imaging
    Microscopy Fluorescence
    Color of Fluorescence Orange
    Cellular Stain Endosomes, Lysosomes
Additional Info

    Details

    VectaCell™ Acridine Orange is a fluorescent dye that can be used to stain acidic organelles, such as lysosomes, autosomes or yeast vacuoles. At low pH (inside the organelles), it will emit an orange fluorescence (peak at 590 nm). For optimal endosome visualization use a blue light excitation (475 nm).

    VectaCell™ Acridine Orange also binds nucleic acids. When bound to double-stranded DNA, it emits a green fluorescence (525 nm); when bound to RNA it fluoresces red (650 nm). For optimal DNA visualization use a 502 nm excitation. For optimal RNA visualization excite at 460 nm.

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Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    SDS Product Specification Sheet