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Amplified Detection

Biotin/avidin or biotin/streptavidin detection systems offer the greatest sensitivity by signal amplification and the greatest flexibility in detection methods. The VECTASTAIN® ABC-AmP™ kits, for example, are complete detection systems that offer sensitivity, reliability, and versatility. Streptavidin or avidin based reagents can detect a variety of macromolecules on the blot: biotinylated secondary antibodies, biotinylated antibodies against fusion protein tags, biotinylated lectins, and even biotinylated target proteins blotted directly onto the membrane.

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