Home / Products / Blot and Gel Detection Blot and Gel Detection Purification, characterization, and identification of proteins or nucleic acids often require analysis of the target in blotting applications. Proteins or nucleic acids are usually separated by size using gel electrophoresis and transferred to a membrane with a blotting technique. A specific protein can be identified from a mixture using different detection methods. Primary antibodies can be used to recognize unique epitopes or genetically engineered fusion protein tags. Lectins with specificities to certain carbohydrate residues can be used to probe for a particular glycoprotein. These reagents can then be detected and visualized with an enzymatic or fluorescent detection method. Nucleic acid blotting applications also require high specificity which is derived from the uniqueness of the target sequence and the fidelity of the complementary probe. The target sequence is detected by a complementary probe labeled with tags such as biotin, digoxigenin (DIG), DNP, or fluorescein. The label is then detected with an antibody to the tag and subsequent detection reagents. Detection sensitivity for both protein and nucleic acid blotting applications depends on the abundance of the target and the quality of the reagents available for detecting the primary antibody or hybridized probe. Showing 1 to 30 of 162 results Position Name Catalog Number Price View as: List Grid Show 30 60 120 Page: 1 2 3 4 5 10x Casein Solution Catalog Number: SP-5020 Unit Size: 250 ml AEC Peroxidase (HRP) Substrate Kit, 3-amino-9-ethylcarbazole Catalog Number: SK-4200 Unit Size: 1 kit Alkaline Phosphatase Anti-DNP Catalog Number: MB-3100 Unit Size: 150 µg Alkaline Phosphatase Goat Anti-Rabbit IgG Antibody Catalog Number: AP-1000 Unit Size: 1 ml Alkaline Phosphatase Horse Anti-Mouse IgG Antibody Catalog Number: AP-2000 Unit Size: 1 ml Animal-Free Blocker (5x) Catalog Number: SP-5030 Unit Size: 250 ml Anti-DNP (Dinitrophenyl), Unconjugated Catalog Number: SP-0603 Unit Size: 1 mg Avidin/Biotin Blocking Kit Catalog Number: SP-2001 Unit Size: 1 kit BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit, 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium Catalog Number: SK-5400 Unit Size: 1 kit Biotinylated Agaricus Bisporus Lectin (ABL) Catalog Number: B-1425 Unit Size: 1 mg Biotinylated Aleuria Aurantia Lectin (AAL) Catalog Number: B-1395 Unit Size: 1 mg Biotinylated Anti-DNP Catalog Number: BA-0603 Unit Size: 0.5 mg Biotinylated Anti-Fluorescein Catalog Number: BA-0601 Unit Size: 0.5 mg Biotinylated Anti-GFP (Anti-Green Fluorescent Protein) Catalog Number: BA-0702 Unit Size: 0.25 mg Biotinylated Anti-Rhodamine Catalog Number: BA-0605 Unit Size: 0.5 mg Biotinylated Concanavalin A (Con A) Catalog Number: B-1005 Unit Size: 5 mg Biotinylated Datura Stramonium Lectin (DSL) Catalog Number: B-1185 Unit Size: 2 mg Biotinylated Dolichos Biflorus Agglutinin (DBA) Catalog Number: B-1035 Unit Size: 5 mg Biotinylated Erythrina Cristagalli Lectin (ECL, ECA) Catalog Number: B-1145 Unit Size: 5 mg Biotinylated Galanthus Nivalis Lectin (GNL) Catalog Number: B-1245 Unit Size: 2 mg Biotinylated Goat Anti-Cat IgG Antibody Catalog Number: BA-9000 Unit Size: 1.5 mg Biotinylated Goat Anti-Chicken IgY Antibody Catalog Number: BA-9010 Unit Size: 1.5 mg Biotinylated Goat Anti-Guinea Pig IgG Antibody Catalog Number: BA-7000 Unit Size: 1.5 mg Biotinylated Goat Anti-Hamster IgG Antibody Catalog Number: BA-9100 Unit Size: 1.5 mg Biotinylated Goat Anti-Horse IgG Antibody Catalog Number: BA-8000 Unit Size: 1.5 mg Biotinylated Goat Anti-Human IgE Antibody, epsilon chain specific Catalog Number: BA-3040 Unit Size: 0.5 mg Biotinylated Goat Anti-Human IgG Antibody Catalog Number: BA-3000 Unit Size: 1.5 mg Biotinylated Goat Anti-Human IgG Antibody, gamma chain specific Catalog Number: BA-3080 Unit Size: 0.5 mg Biotinylated Goat Anti-Human IgM Antibody, mu chain specific Catalog Number: BA-3020 Unit Size: 0.5 mg Biotinylated Goat Anti-Human Kappa Chain Antibody Catalog Number: BA-3060 Unit Size: 0.5 mg Showing 1 to 30 of 162 results Position Name Catalog Number Price View as: List Grid Show 30 60 120 Page: 1 2 3 4 5 Didn't find what you were looking for? Submit a suggestion.