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ImmPACT DAB Peroxidase (HRP) Substrate

Catalog Number: SK-4105

Availability: In stock

Unit Size: 120 ml

Prostate:  Prostate Specific Antigen (m), ImmPRESS Reagent (HRP), ImmPACT DAB (brown). Zoom

Overview

    CE markedFeatures:

    • 2-4 fold greater sensitivity than original substrate kit
    • Optimized for IHC/ICC/ISH and blots
    • Heat Stable
    • Permanent mounting
    • Ideal for single and multiple labeling
    • Working solutions stable for up to 14 days at 4 °C
    • One year expiry date
    • Supplied as 120 ml of diluent and concentrated DAB stock solution

    Additional Information

    Catalog Number SK-4105
    Unit Size 120 ml
    CE Marked Yes
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
    Detection Enzyme(s) Peroxidase
    Substrate Reaction Product Precipitating Reaction Product
    Chromogen Color Brown
    Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
    Additional Substrate Properties Heat Resistant, Multiple Labelling
    Mounting Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)
Additional Info

    Details

    DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted. The DAB reaction product can be intensified with DAB Enhancing Solution (H-2200).

    This product is an entirely new formula for a diaminobenzidine based peroxidase substrate. ImmPACT™ DAB produces a dark brown reaction product that is crisper and is 3-4 times more sensitive than the original Vector DAB substrate kit. It can be used for both manual and automated staining methods. The ImmPACT™ DAB working solution is stable for at least five days at room temperature and at least two weeks when stored at 4° C. This product consists of 120 ml of diluent and a convenient concentrated stock solution of highly purified DAB. This product can also be used on blots.

    Relative Sensitivity of Substrates

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