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ImmPACT DAB EqV Peroxidase (HRP) Substrate

Catalog Number: SK-4103

Availability: In stock

Unit Size: 400 ml

Prostate:  Anti-PSA (m), ImmPRESS (HRP) Anti-Mouse Ig Kit, ImmPACT DAB EqV (brown) substrate. Zoom

Overview

    Features:

    • 3 - 5 times more sensitive than the original DAB substrate
    • Optimized for IHC/ICC/ISH
    • Heat Stable
    • Permanent mounting
    • Ideal for single and multiple labeling
    • Can be used for blot detection on nylon and PVDF membranes but is not recommended for nitrocellulose membranes
    • Working solution is stable for several days when stored at 4 °C
    • One year expiry date
    • Consists of 200 ml each of Reagent 1 and Reagent 2 which when mixed together in equal volumes form the working solution

    Additional Information

    Catalog Number SK-4103
    Unit Size 400 ml
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
    Detection Enzyme(s) Peroxidase
    Substrate Reaction Product Precipitating Reaction Product
    Chromogen Color Brown
    Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
    Additional Substrate Properties Heat Resistant, Multiple Labelling
    Mounting Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)
Additional Info

    Details

    DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted. The DAB reaction product can be intensified with DAB Enhancing Solution (H-2200).

    ImmPACT™ DAB EqV (equal volume) consists of 200 ml each of Reagent 1 and Reagent 2 which when mixed together in equal volumes form the working solution. ImmPACT™ DAB EqV produces a dark brown reaction product that is 3 to 5 times more sensitive than the original DAB substrate. The working solution is stable for several days when stored at 4 °C. This substrate can be used for both manual and automated staining methods. ImmPACT™ DAB EqV can be used for blot detection on nylon and PVDF membranes but is not recommended for nitrocellulose membranes.

    Relative Sensitivity of Substrates

    Substrate Combinations

     

    Counterstain Compatibility

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