Horse Anti-Mouse IgG Antibody (H+L), Texas Red®

TI-2000
SKU Unit Size Price Qty
TI-2000-1.5 1.5 mg
$164.00

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Description

Texas Red® Horse Anti-Mouse IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L) 
  • Optimally labeled with Texas Red® to provide the brightest label for fluorescence microscopy 
  • Supplied in solution 
  • Excitation: 595 nm 
  • Emission: 615 nm 
  • Color: Red

Specifications

More Information
Unit Size 1.5 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 606-615 nm
Maximum Excitation 595-604 nm
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2 % normal serum from the same species as the tissue.
Target Species Mouse
Conjugate Texas Red
Color of Fluorescence Red
Host Species Horse
Format Concentrate

Documents

Technical Information

The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 

Texas Red® (sulforhodamine 101) produces a bright red fluorescence well removed from the emission spectrum of fluorescein. Texas Red® Horse Anti-Mouse IgG Antibody conjugate is ideal as a single or double label reagent for flow cytometry or tissue staining.