Streptavidin, Phycoerythrin (SAPE)

SA-5207
SKU Unit Size Price Qty
SA-5207-1 1 mg
$173.00

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Phycoerythrin is a fluorescent protein that produces a bright red-orange fluorescence
  • Can be used in cell sorting and in tissue staining
  • Highly purified and possesses very low non-specific binding properties
  • Excitation at 565 nm
  • Emission at 574 nm
  • Color:  Red-Orange

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, Flow Cytometry/Cell Separation
Recommended Usage Recommended Working Dilution: 10 µg/ml
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 450-580 nm
Maximum Emission 570-590 nm
Recommended Storage 2-8 °C
Concentration 1 mg/ml
Conjugate Phycoerythrin
Color of Fluorescence Red-Orange
Format Concentrate

Documents

Technical Information

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

Our newly improved Phycoerythrin Streptavidin (SA-5207) is substantially brighter than previous conjugates.  Phycoerythrin Streptavidin is a highly fluorescent label which can overcome problems of endogenous fluorescence.

If this conjugate is used in tissue staining, VECTASHIELD® HardSet™ or Vibrance Mounting Medium is recommended for mounting. Optimal signal characteristics are maintained for 24 hours following mounting.