SHNH (Succinimidyl 6-hydraziniumnicotinate hydrochloride) is used to incorporate HyNic (6-hydrazinonicotinamide) linkers on proteins, peptides and other amine-containing molecules for Technetium-99m (Tc-99m) labeling. SHNH is a bifunctional linker that incorporates HyNic through amino groups on biomolecules via an NHS ester. HyNic groups were initially developed to link Tc-99m to peptides and proteins for in vivo imaging. This procedure is still used in tens of millions of medical diagnostic procedures annually, making it the most commonly used medical radioisotope.
| Label/Modifier Type | HyNic |
|---|---|
| Reactivity | 4FB |
| Recommended Storage | Desiccated: -15° to -25°C |
| Applications | Antibody Labeling, Antisense/RNAi, Aptamers |
| Other Name(s) | SoluLINK Bioconjugation |
SHNH is an bifunctional aromatic hydrazine linker used to incorporate HyNic (6-hydrazinonicotinamide) linkers onto biomolecules through their amino group via an activated ester (i.e. NHS; Figure 1). HyNic groups were developed to link Tc-99m to proteins for in vivo imaging.
Figure 1: Scheme presenting the modification of a protein with SHNH.
The number of HyNic linkers incorporated on biomolecules can be quantified colorimetrically on reaction with 2-sulfobenzaldehyde (catalog number S-2005-100). The product yields a chromophore that absorbs at A350 with a molar extinction coefficient of 28,500 L/(mol*cm).
SoluLINK® Bioconjugation For Research Use Only. Not for use in diagnostic procedures. For additional licensing restrictions, please see the license agreement at vectorlabs.com/solulink-research-license.
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