Rhodamine Avidin DCS, Cell Sorting Grade

A-2012
SKU Unit Size Price Qty
A-2012-1 1 mg
$158.00

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Description

Avidin DCS, Rhodamine labeled, is a cell sorter grade of Rhodamine Avidin D. Through the development of a new method of labeling we are able to incorporate more fluorochrome into a protein without altering the properties of the protein. Not only does the Rhodamine Avidin DCS have a high F/P ratio, the efficiency of fluorescence is also increased. In addition to the improved conjugation technique, we employ a purification step to reduce nonspecific binding which can hamper resolution in cell sorting and other applications. 

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage For diluting this product, we recommend a HEPES- or bicarbonate-buffered saline solution, approximately pH 8.2. The recommended range of concentrations for staining of cells or tissues is 5-30 µg/ml.
Solution 10 mM HEPES, 0.5 M NaCl, pH 7.5, 0.08% sodium azide, 10 mg/ml beta cyclodextrin
Maximum Excitation 550-560 nm
Maximum Emission 568-575 nm
Recommended Storage 2-8 °C
Concentration 2.0 mg active conjugate/ml
Conjugate Rhodamine
Color of Fluorescence Red
Format Concentrate

Documents

Technical Information

Although this product has been designed for cell sorting, it can also be used in fluorescent microscopy when higher sensitivity and lower background staining are necessary. This derivative is preferred when using the amplification procedure with Biotinylated Anti-Avidin D. 
 
  • Excitation maximum:  550 nm
  • Emission maximum:  575 nm
  • Color:  Red