R-Phycoerythrin Horse Anti-Mouse IgG Antibody

EI-2007
SKU Unit Size Price Qty
EI-2007-1 1 mg
$183.00

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Description

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
  • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L) 
  • Optimally labeled with R-Phycoerythrin to provide the brightest label for fluorescence microscopy 
  • Supplied in solution 
  • Excitation: 565 nm 
  • Emission: 574 nm 
  • Color: Red-Orange

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, Flow Cytometry/Cell Separation
Concentration 1 mg active conjugate/ml
Recommended Storage 2-8 °C. DO NOT FREEZE
Solution 10 mM HEPES, 150 mM NaCl, pH 7.4, 0.08% sodium azide.
Maximum Emission 570-590 nm
Maximum Excitation 450-580 nm
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Target Species Mouse
Conjugate Phycoerythrin
Color of Fluorescence Red-Orange
Host Species Horse
Format Concentrate

Documents

Technical Information

The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. Unless otherwise specified, our antibodies will recognize both heavy and light chains (H+L).

R-Phycoerythrin Horse Anti-Mouse IgG Antibody is labeled optimally with R-phycoerythrin and is intended for use in flow cytometry or tissue staining, employing single or dual labeling systems.

If this antibody is used in tissue staining, VECTASHIELD® HardSet™ Mounting Medium is recommended for mounting. Optimal signal characteristics are maintained for 24 hours following mounting.