ProtOn HRP (Peroxidase) Antibody and Protein Labeling Kit

Cat. No: PLK-1203
Price: $322.00
Unit Size: 1 kit

Overview

Features:

  • The ProtOn™ HRP Protein Labeling Kit is designed for simple and fast labeling of proteins with Horseradish Peroxidase (HRP).
  • The kit is ideally suited to label antibodies or other proteins.
  • HRP is activated under precise conditions to generate an optimal number of reactive aldehyde groups for subsequent conjugation with amine-containing molecules via reductive amination.
  • Conjugation of the HRP is accomplished through reaction with primary amine on lysine residues or terminal amino groups on target proteins to form a stable secondary amine linkage. 

Kit Contents:

Each kit contains all the components needed for at least five labeling reactions.

  • Activated HRP Lyophilized Powder
  • Reaction Buffer (150 mM NaHCO3, pH 9.5 with preservative)
  • Reduction Buffer (5M Sodium Cyano- borohydride solution)
  • Quenching Buffer (1M Glycine with preservative) 

Each ProtOn™ Kit is supplied with complete instructions that offer the researcher a step-by-step guide to convenient and easy protein labeling with HRP.

 Vector ProtOn HRP Protein and Antibody Labeling Kit

 Prostate (FFPE):  Rabbit Anti-PSA primary antibody directly labeled with HRP using Vector ProtOn HRP Protein Labeling Kit (left) or using competitor TF HRP protein labeling kit (right).  Note the more intense and crisp stain achieved when using the primary antibody labeled with Vector ProtOn HRP Protein Labeling Kit (left).

 

Cat. No. PLK-1203
Unit Size 1 kit
Country of Manufacture United States
Target for Labeling Protein
Required Reactive Group Primary Amino Group
Tag/Group Incorporated HRP (Peroxidase)

More Information

Protein labeling and modification is a powerful tool for the isolation and analysis of proteins. The ProtOn™ Protein Labeling Kits simplify the attachment of biotin, fluorescein, or peroxidase (HRP) to antibodies or other proteins. Vector Laboratories offers a variety of reagents and kits for protein labeling allowing for greater flexibility of experimental design.

The ProtOn HRP Protein Labeling Kit is designed for simple and fast labeling of proteins with Horseradish Peroxidase (HRP). The kit is ideally suited to label antibodies or other proteins.  HRP is activated under precise conditions to generate an optimal number of reactive aldehyde groups for subsequent conjugation with amine-containing molecules via reductive amination. Conjugation of the HRP is accomplished through reaction with primary amine on lysine residues or terminal amino groups on target proteins to form a stable secondary amine linkage.

Kit Contents:

Each kit contains all the components needed for at least five labeling reactions.

  • Activated HRP Lyophilized Powder
  • Reaction Buffer (150 mM NaHCO3, pH 9.5 with preservative)
  • Reduction Buffer (5M Sodium Cyano- borohydride solution)
  • Quenching Buffer (1M Glycine with preservative) 

Each ProtOn™ Kit is supplied with complete instructions that offer the researcher a step-by-step guide to convenient and easy protein labeling with HRP.

Protocols/Data Sheets/SDS