Shopping Cart (0)
$0.00

You have no items in your shopping cart.

ProtOn HRP (Peroxidase) Antibody and Protein Labeling Kit

Catalog Number: PLK-1203

Availability: In stock

$295.00
Unit Size: 1 kit

Prostate (FFPE):  Rabbit Anti-PSA primary antibody directly labeled with HRP using Vector ProtOn HRP Labeling Kit, followed by Vector DAB Substrate Kit. Zoom

Overview

    Features:

    • The ProtOn™ HRP Protein Labeling Kit is designed for simple and fast labeling of proteins with Horseradish Peroxidase (HRP).
    • The kit is ideally suited to label antibodies or other proteins.
    • HRP is activated under precise conditions to generate an optimal number of reactive aldehyde groups for subsequent conjugation with amine-containing molecules via reductive amination.
    • Conjugation of the HRP is accomplished through reaction with primary amine on lysine residues or terminal amino groups on target proteins to form a stable secondary amine linkage. 

    Kit Contents:

    Each kit contains all the components needed for at least five labeling reactions.

    • Activated HRP Lyophilized Powder
    • Reaction Buffer (150 mM NaHCO3, pH 9.5 with preservative)
    • Reduction Buffer (5M Sodium Cyano- borohydride solution)
    • Quenching Buffer (1M Glycine with preservative) 

    Each ProtOn™ Kit is supplied with complete instructions that offer the researcher a step-by-step guide to convenient and easy protein labeling with HRP.

     Vector ProtOn HRP Protein and Antibody Labeling Kit

     Prostate (FFPE):  Rabbit Anti-PSA primary antibody directly labeled with HRP using Vector ProtOn HRP Protein Labeling Kit (left) or using competitor TF HRP protein labeling kit (right).  Note the more intense and crisp stain achieved when using the primary antibody labeled with Vector ProtOn HRP Protein Labeling Kit (left).

     

    Additional Information

    Catalog Number PLK-1203
    Unit Size 1 kit
    Country of Manufacture United States
    Target for Labeling Protein
    Required Reactive Group Primary Amino Group
    Tag/Group Incorporated HRP (Peroxidase)
Additional Info

    Details

    Protein labeling and modification is a powerful tool for the isolation and analysis of proteins. The ProtOn™ Protein Labeling Kits simplify the attachment of biotin, fluorescein, or peroxidase (HRP) to antibodies or other proteins. Vector Laboratories offers a variety of reagents and kits for protein labeling allowing for greater flexibility of experimental design.

    The ProtOn HRP Protein Labeling Kit is designed for simple and fast labeling of proteins with Horseradish Peroxidase (HRP). The kit is ideally suited to label antibodies or other proteins.  HRP is activated under precise conditions to generate an optimal number of reactive aldehyde groups for subsequent conjugation with amine-containing molecules via reductive amination. Conjugation of the HRP is accomplished through reaction with primary amine on lysine residues or terminal amino groups on target proteins to form a stable secondary amine linkage.

    Kit Contents:

    Each kit contains all the components needed for at least five labeling reactions.

    • Activated HRP Lyophilized Powder
    • Reaction Buffer (150 mM NaHCO3, pH 9.5 with preservative)
    • Reduction Buffer (5M Sodium Cyano- borohydride solution)
    • Quenching Buffer (1M Glycine with preservative) 

    Each ProtOn™ Kit is supplied with complete instructions that offer the researcher a step-by-step guide to convenient and easy protein labeling with HRP.

Reviews

    Write Your Own Review

    You're reviewing: ProtOn HRP (Peroxidase) Antibody and Protein Labeling Kit

Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    SDS Protocol