ProtOn Fluorescein Antibody and Protein Labeling Kit

Cat. No: PLK-1201
Price: $305.00
Unit Size: 1 kit

Overview

Features:

The ProtOn™ Fluorescein Labeling Kit is designed for simple and fast labeling of proteins with fluorescein.

The kit is ideally suited to label antibodies, hormones, or other proteins as well as other macromolecules that have primary amino groups.

Labeled proteins can be used in a number of applications, such as immunological assays, fluorescence microscopy, investigation of protein-protein interactions, etc.

Each kit contains all the components needed for at least five labeling reactions (up to 1 mg of protein per reaction).

Kit components:

  • Fluorescein Labeling Reagent (FITC)
  • Reagent Solvent
  • Reaction Buffer
  • Stop Reagent
  • Gel Filtration Slurry
  • 5 Spin Columns
  • 5 Collection Tubes
Cat. No. PLK-1201
Unit Size 1 kit
Country of Manufacture United States
Target for Labeling Protein
Required Reactive Group Primary Amino Group
Tag/Group Incorporated Fluorescein

More Information

Protein labeling and modification is a powerful tool for the isolation and analysis of proteins. The ProtOn™ Protein Labeling Kits simplify the attachment of either biotin or fluorescein to antibodies or other proteins. Vector Laboratories offers a variety of reagents and kits for protein labeling allowing for greater flexibility of experimental design.

This ProtOn™ protein labeling kit is designed for the simple and fast labeling of proteins with fluorescein. The kit is ideally suited to label antibodies, hormones, or other proteins as well as other macromolecules containing primary amino groups. Labeled proteins can be used in a number of applications, such as immunological assays, fluorescence microscopy, investigation of protein-protein interactions, etc.

Kit Contents:

Each kit contains all the components needed for at least five labeling reactions (up to 1 mg of protein per reaction). Kit components include:

  • Labeling Reagent (fluorescein isothiocyanate)
  • Reagent Solvent
  • Reaction Buffer
  • Stop Reagent
  • Gel Filtration Slurry
  • 5 x 1 ml Spin Columns
  • 5 x 2 ml Collection Tubes

The ProtOn™ labeling reagents react with primary amines (i.e. lysine residues or terminal amino groups) on the protein, forming a covalent bond. The linkage between the label and the protein produced with these reagents is stable, with no significant loss of the label during storage.

Each ProtOn™ Kit is supplied with complete instructions that offer the researcher a step-by-step guide to convenient and easy protein labeling with biotin or fluorescein.

 

Protocols/Data Sheets/SDS