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ProtOn Biotin Antibody and Protein Labeling Kit

Catalog Number: PLK-1202

Availability: In stock

$290.00
Unit Size: 1 kit

Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN Elite ABC Reagent and Vector DAB substrate. Zoom

Overview

    Features:

    The ProtOn™ Biotin Labeling Kit is designed for simple and fast labeling of proteins with biotin.

    The kit is ideally suited to label antibodies, hormones, or other proteins as well as other macromolecules that have primary amino groups.

    Labeled proteins can be used in a number of applications, such as immunological assays, fluorescence microscopy, investigation of protein-protein interactions, etc.

    Each kit contains all the components needed for at least five labeling reactions (up to 1 mg of protein per reaction).

    Kit components:

    • Labeling Reagent (Biotin [Long Arm] NHS)
    • Reagent Solvent
    • Reaction Buffer
    • Stop Reagent
    • Gel Filtration Slurry
    • 5 Spin Columns
    • 5 Collection Tubes
    • Agarose Avidin D Slurry

    Additional Information

    Catalog Number PLK-1202
    Unit Size 1 kit
    Country of Manufacture United States
    Target for Labeling Protein
    Required Reactive Group Primary Amino Group
    Tag/Group Incorporated Biotin
Additional Info

    Details

    Protein labeling and modification is a powerful tool for the isolation and analysis of proteins. The ProtOn™ Protein Labeling Kits simplify the attachment of either biotin or fluorescein to antibodies or other proteins. Vector Laboratories offers a variety of reagents and kits for protein labeling allowing for greater flexibility of experimental design.

    This ProtOn™ protein labeling kit is designed for the simple and fast labeling of proteins with biotin. The kit is ideally suited to label antibodies, hormones, or other proteins as well as other macromolecules containing primary amino groups. Labeled proteins can be used in a number of applications, such as immunological assays, fluorescence microscopy, investigation of protein-protein interactions, etc.

    Kit Contents:

    Each kit contains all the components needed for at least five labeling reactions (up to 1 mg of protein per reaction). Kit components include:

    • Labeling Reagent (Biotin [Long Arm] NHS)
    • Reagent Solvent
    • Reaction Buffer
    • Stop Reagent
    • Gel Filtration Slurry
    • 5 x 1 ml Spin Columns
    • 5 x 2 ml Collection Tubes
    • Agarose Avidin D Slurry

    The ProtOn™ labeling reagents react with primary amines (i.e. lysine residues or terminal amino groups) on the protein, forming a covalent bond. The linkage between the label and the protein produced with these reagents is stable, with no significant loss of the label during storage.

    Each ProtOn™ Kit is supplied with complete instructions that offer the researcher a step-by-step guide to convenient and easy protein labeling with biotin or fluorescein.

     

     

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