A Brilliant Solution for Fluorescence Signal Retention
Introducing VECTASHIELD® Vibrance® Antifade Mounting Media
VECTASHIELD Vibrance products are completely new formulations of curing antifade mounting media for immunofluorescence applications. These newest additions to our VECTASHIELD portfolio were developed with customer feedback to improve on parameters such as ease of use and retention of specific staining intensity over time. VECTASHIELD Vibrance Antifade Mounting Media are new tools to help investigators see more and do more with each experiment.
Why VECTASHIELD Vibrance Mounting Media?
- Superior antifade/anti-photobleaching properties across the spectrum
- Compatibility with commonly used fluorophores
- View sections one hour after mounting
- No tone or autofluorescent background, even after curing
- Room temperature storage of slides with extended archiving time
- Minimal bubble formation, even after several weeks storage
- Curing formulations with choice of counterstain (DAPI) or no counterstain
- 12 month product expiration date
- Ideal refractive index
- Easy to use
- Ready to use, requires no warming
- No sealing of coverslips required (curing formulations)
Easy Application and Use of VECTASHIELD Vibrance
Excellent Antifade Properties Across the Spectrum, with Commonly Used Fluorophores
VECTASHIELD Vibrance Mounting Media provide excellent protection against fading across the visible spectrum, even under far-red wavelengths. They are compatible with most commercially available fluorophores.
Viewable in as Little as One Hour After Mounting – No Need to Wait 24 Hours
Competitor immunofluorescence workflows recommend slides be viewed 24 hours after mounting. VECTASHIELD Vibrance Mounting Media, however, allow same-day viewing to accelerate discovery without sacrificing signal intensity or retention.
Extended Archiving of Mounted Sections at Room Temperature
Archiving fluorescently stained sections at room temperature often leads to signal reduction and dessication of the specimen. Slides mounted with VECTASHIELD Vibrance Medium, however, can be stored for weeks at ambient temperature, in standard slide boxes, with no loss of specific signal intensity or specimen integrity. The stabilizing effect of VECTASHIELD Vibrance Mounting Medium provides greater flexibility for imaging and storing fluorescently stained samples.
Serial sections of human colon tissue (FFPE) mounted with either VECTASHIELD Vibrance Mounting Medium with DAPI (H-1800, left image) or competitor mounting medium with DAPI (right image), each stored at room temperature for two weeks and then imaged.
Note absence of bubbles and background in blue spectrum in left image using VECTASHIELD Vibrance Mounting Medium with DAPI compared with right image, after two weeks at room temperature.
…with No Specimen Obscuring Retraction or Bubble Formation
Another problem commonly observed in immunoflourescence staining is the formation over time of bubbles under the coverslip, or shrinking (retraction) of the media. This phenomenon detracts from the overall quality of the sections and can obscure specific staining. The unique formulation of VECTASHIELD Vibrance Mounting Medium provides a uniform sheet of medium under the coverslip, with essentially no bubble formation or retraction—even after many weeks of storage at room temperature.
Serial sections of human colon tissue (FFPE) stained for cytokeratin (AE1/AE3, mouse primary) followed by Alexa Fluor™ 488 anti-mouse IgG secondary antibody. Sections were mounted with either VECTASHIELD Vibrance Mounting Medium (H-1700, left image) or competitor mounting medium (right image) stored at room temperature for two weeks, and then imaged.
Note absence of bubbles and background in green spectrum in left image using VECTASHIELD Vibrance Mounting Medium compared with right image, after two weeks at room temperature.
Publications:
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Ghinassi, B. et al. 2020. Immunohistochemical Results of Soft Tissues around a New Implant Healing-Abutment Surface: A Human Study. J. Clin. Med. 9(4), 1009 (https://www.mdpi.com/2077-0383/9/4/1009/htm)
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Steeves, A. et al. 2020. The Implication of Spatial Statistics in Human Mesenchymal Stem Cell Response to Nanotubular Architectures. Int J Nanomedicine. 15: 2151–2169. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125340/)
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Crapster, A. et al. (2020) HIPK4 is essential for murine spermiogenesis. eLife;9:e50209. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067585/)
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Tsakmaki, A. et al. (2020) ISX-9 manipulates endocrine progenitor fate revealing conserved intestinal lineages in mouse and human organoids. Molecular Metabolism. 34: 157e173. (https://www.sciencedirect.com/science/article/pii/S221287782030020X)
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Jacob, F. et al. (2020) A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity. Cell (https://doi.org/10.1016/j.cell.2019.11.036)
- Demine, S. et al. (2020) Pro-inflammatory cytokines induce cell death, inflammatory responses, and endoplasmic reticulum stress in human iPSC-derived beta cells. Stem Cell Research & Therapy (https://link.springer.com/article/10.1186/s13287-019-1523-3)
- de Moura, E.G.H. et al. (2019) Histologic assessment of the intestinal wall following duodenal mucosal resurfacing (DMR): a new procedure for the treatment of insulin-resistant metabolic disease. Endosc Int Open. (https://www.thieme-connect.com/products/ejournals/html/10.1055/a-0862-0263)
- Steinbichler, T. B. et al. (2019) Pleiotropic effects of epithelial mesenchymal crosstalk on head and neck cancer: EMT and beyond. Cancer Microenvironment (https://link.springer.com/article/10.1007%2Fs12307-019-00228-y)
- Zelek, W.M. et al. (2019) Development and characterization of novel anti-C5 monoclonal antibodies capable of inhibiting complement in multiple species. Immunology (https://onlinelibrary.wiley.com/doi/full/10.1111/imm.13083)
- Wang L. et al. (2019) Designable nanoplasmonic biomarkers for direct microscopy cytopathology diagnostics. J. Biophotonics. (https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201900166)
- Mendoza-Torreblanca, J.G. et al. (2019) Analysis of Differential Expression of Synaptic Vesicle Protein 2A in the Adult Rat Brain. Neuroscience (https://www.sciencedirect.com/science/article/abs/pii/S0306452219306499?via%3Dihub)
- Leskelä, S. et al. (2019) C9orf72 Proteins Regulate Autophagy and Undergo Autophagosomal or Proteasomal Degradation in a Cell Type-Dependent Manner. Cells (https://www.mdpi.com/2073-4409/8/10/1233)
- Castillo-Martin, M. et al. (2019) Transformed Bone Marrow Cells Generate Neoplasms of Distinct Histogenesis. A Murine Model of Cancer Transplantation. Stem Cell Research (https://www.sciencedirect.com/science/article/pii/S1873506119302673#!)
- Wong, W. et al. (2019) The exercise cytokine interleukin 15 rescues slow wound healing in aged mice. Journal of Biological Chemistry (http://www.jbc.org/content/early/2019/11/20/jbc.RA119.010740)
Customer Testimonials
Ordering Information
| Product | Unit Size | No Counterstain | With DAPI Counterstain | With PI Counterstain | TRITC-Phalloidin |
|
VECTASHIELD Vibrance Antifade Mounting Medium (hardening) |
2ml, 10ml | H-1700 | H-1800 | ||
|
VECTASHIELD PLUS Antifade Mounting Medium |
2ml, 10ml | H-1900 | H-2000 | ||
|
VECTASHIELD Antifade Mounting Medium (non-hardening) |
10ml | H-1000-10 | H-1200-10 | H-1300-10 | |
|
VECTASHIELD Hardset™ Antifade Mounting Medium (hardening) |
10ml | H-1400-10 | H-1500-10 | H-1600-10 |