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UBQ-1 NHS Ester (FP-1731)

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Description

Universal Black 1 Quencher (UBQ-1, Black Hole Quencher®-2 (BHQ®-1) equivalent) is a dark quencher with no native emission due to the polyaromatic-azo backbone. This nonfluorescent acceptor is often the quencher of choice for qPCR probes and for other fluorescence-quenched probe applications. A broad and intense quenching range from 480-580 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with green to yellow emitting dyes such as FAM, Oregon Green®, TET, JOE and HEX, Alexa Fluor® 488 and DyLight® 488.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

Specifications

Unit Size5 mg, 25 mg, 100 mg
Reactivity
Primary amine
Abs/Em Maxima534 nm
Extinction coefficient34,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesFAM, Oregon Green®, TET, JOE and HEX, Alexa Fluor® 488 and DyLight® 488
Molecular weight586.58
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

UB 2

Protein Labeling Calculator

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