Whilst biotin is a highly effective affinity label, fluorescent labels provide a more sensitive, quantitative, and convenient method for visualizing proteins. Trifunctional click chemistry probes that incorporate a ligation handle, a biotin and a fluorophore straightforwardly extended to combine the complementary benefits of both types of label. Dual-label TAMRA Biotin Azide probe can be readily incorporated into alkyne-tagged biomolecules through CuAAC ligation. For example, dual-labeled newly synthesized proteins, glycans, lipids, or DNA/RNA can be readily isolated by affinity purification and visualized by in-gel fluorescence.
Another very important advantage of dual label probes over regular biotin probes is built-in control. Each step of enrichment process can easily followed either by UV-Vis (550 nm) or by more sensitive fluorescence spectroscopy. After elution form streptavidin beads target proteins containing TAMRA label can be easily distinguished from non-specifically bound proteins and endogenously biotinlytated proteins.
This new dual label probe should be useful to expand the new applications of standard biotin–streptavidin technology.
Unit Size | 1 mg, 5 mg, 25 mg |
---|---|
Molecular weight | 1174.37 |
Chemical composition | C57H79N11O14S |
CAS | N/A |
Solubility | DMSO, DMF |
Appearance | Red solid |
Storage Conditions | -20°C. |
Shipping Conditions | Ambient temperature |
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