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Vector® Red Substrate Kit, Alkaline Phosphatase (AP) (SK-5100)

Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS-AP Reagent,
Prostate (double label): Cytokeratin (m), VECTASTAIN ABC-AP Kit, Vector Blue (blue) substrate. CD34, VECTASTAIN ABC-AP Kit, Vector Red (red) substrate.
Uterus (double label): Epithelium (blue, Vector Blue), muscle (red, Vector Red).
Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting
Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting
Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting
100302 9vreddapimod 11 jpg
Melanoma was stained with anti-vimentin followed by ImmPRESS™-AP Anti-Rabbit IgG Reagent and Vector Red Substrate. Note the excellent contrast of the substrate with the brown pigments.
Melanoma was stained with anti-vimentin followed by ImmPRESS™-AP Anti-Rabbit IgG Reagent and Vector Red Substrate. Viewed under fluorescence microscopy.
Lymph Node (double label): Ki67 (m), VECTASTAIN Universal ABC-AP Kit, Vector Red Substrate (red); Multi-cytokeratin (m), VECTASTAIN Universal Elite ABC Kit, DAB substrate (brown).
Tumor (double label): CD34 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue AP substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red).
Tumor (double label): p53 protein (m), VECTASTAIN ABC-AP Kit, Vector Red substrate (red); Pan-Cytokeratin (m), VECTASTAIN Elite ABC Kit, Vector SG Substrate (blue/gray).
Breast Carcinoma (double label): Estrogen Receptor (m), ImmPRESS Universal Reagent, Vector DAB substrate (brown); Multi-Cytokeratin (m), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red); CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector DAB substrate (brown).
Vector® Red Substrate Kit, Alkaline Phosphatase (AP)
Small Bowel (frozen, double label): PGP9.5 (m), MOM Basic Kit, VECTASTAIN ABC AP Standard Kit, Vector Red substrate (red); Desmin (m), MOM Fluorescein Kit (green).
Breast Carcinoma (frozen, double label): Estrogen Receptor (m), MOM Fluorescein Kit (green); Multi-Cytokeratin (m), MOM Basic Kit, VECTASTAIN ABC-AP Standard Kit, Vector Red AP substrate (red).
In situ hybridization using FastTag FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red Substrate. Mounted in VECTASHIELD Mounting Media with DAPI.
Vector® Red Substrate Kit, Alkaline Phosphatase (AP)
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS-AP Reagent,
Prostate (double label): Cytokeratin (m), VECTASTAIN ABC-AP Kit, Vector Blue (blue) substrate. CD34, VECTASTAIN ABC-AP Kit, Vector Red (red) substrate.
Uterus (double label): Epithelium (blue, Vector Blue), muscle (red, Vector Red).
Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting
Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting
Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting
100302 9vreddapimod 11 jpg
Melanoma was stained with anti-vimentin followed by ImmPRESS™-AP Anti-Rabbit IgG Reagent and Vector Red Substrate. Note the excellent contrast of the substrate with the brown pigments.
Melanoma was stained with anti-vimentin followed by ImmPRESS™-AP Anti-Rabbit IgG Reagent and Vector Red Substrate. Viewed under fluorescence microscopy.
Lymph Node (double label): Ki67 (m), VECTASTAIN Universal ABC-AP Kit, Vector Red Substrate (red); Multi-cytokeratin (m), VECTASTAIN Universal Elite ABC Kit, DAB substrate (brown).
Tumor (double label): CD34 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue AP substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red).
Tumor (double label): p53 protein (m), VECTASTAIN ABC-AP Kit, Vector Red substrate (red); Pan-Cytokeratin (m), VECTASTAIN Elite ABC Kit, Vector SG Substrate (blue/gray).
Breast Carcinoma (double label): Estrogen Receptor (m), ImmPRESS Universal Reagent, Vector DAB substrate (brown); Multi-Cytokeratin (m), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red); CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector DAB substrate (brown).
Vector® Red Substrate Kit, Alkaline Phosphatase (AP)
Small Bowel (frozen, double label): PGP9.5 (m), MOM Basic Kit, VECTASTAIN ABC AP Standard Kit, Vector Red substrate (red); Desmin (m), MOM Fluorescein Kit (green).
Breast Carcinoma (frozen, double label): Estrogen Receptor (m), MOM Fluorescein Kit (green); Multi-Cytokeratin (m), MOM Basic Kit, VECTASTAIN ABC-AP Standard Kit, Vector Red AP substrate (red).
In situ hybridization using FastTag FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red Substrate. Mounted in VECTASHIELD Mounting Media with DAPI.
Vector® Red Substrate Kit, Alkaline Phosphatase (AP)
[text-backorder]

Description

Vector® Red AP Substrate Kit produces a magenta heat stable reaction product that is also fluorescent. Sections stained with the Vector® Red substrate can be dehydrated, cleared, and permanently mounted.

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Ideal for single and multiple labeling
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • One year expiry date
  • Sufficient reagents to produce 200 ml of working solution
  • Excitation: broad excitation spectrum, approx. 365 nm to 560 nm
  • Emission: Above 560 nm
  • Color: Red

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s)Alkaline Phosphatase
Substrate Reaction ProductPrecipitating Reaction Product
Chromogen ColorMagenta
Color of FluorescenceRed
MicroscopyBrightfield, Fluorescence, Spectral Imaging
Additional Substrate PropertiesContrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
MountingAqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)

Kit Contents:

  • 4 ml Vector Red Reagent 1
  • 4 ml Vector Red Reagent 2
  • 4 ml Vector Red Reagent 3

Documents

Product FAQs

Vector Red substrate kit (SK-5100) does require a Tris buffer with a minimum of 100 mM to be present for a reaction to satisfactorily occur. It is recommended to use a 200 mM Tris buffer at pH 8.2-8.5 for the most optimal staining results. If a buffer with < 100 mM Tris is used to make up the Vector Red working solution it may be that no reaction will occur.

Citations

Technical Information

This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. Because of its stability under ISH conditions, Vector® Red can be used in IHC/ISH double labeling applications. 

The bright red to orange fluorescence of the Vector Red reaction product can be visualized using DyLight™ 594, Texas Red® or TRITC filter systems. This fluorescence is especially bright in dehydrated, permanently mounted sections. The fluorescence is stable and does not photobleach like most fluorescent dyes. This substrate can also be used for in situ hybridization or on blots, where it has a wider dynamic range than other substrates.

This kit contains stock solutions in convenient dropper bottles.

SUBSTRATEsensitivity2 jpg

Substrate.CounterstainChart jpg SubstrateCombos jpg

 

Applicable patents and legal notices are available at legal notices.

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