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ImmPACT® SG Substrate Kit, Peroxidase (HRP) (SK-4705)

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Description

The ImmPACT SG HRP substrate produces a blue-gray reaction product that can be used as a single label or as a second color for multiple antigen labeling. It is 2-4 fold greater sensitivity than original substrate kit.

Features:

  • Optimized for IHC/ICC/ISH and blots
  • Permanent mounting
  • Working solutions stable for up to 14 days at 4 °C
  • Supplied as 120 ml of diluent and stock solutions in dropper bottles

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s)Peroxidase
Substrate Reaction ProductPrecipitating Reaction Product
Chromogen ColorBlue-Gray
MicroscopyBrightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate PropertiesContrast with Tissue Pigments, Multiple Labeling
MountingAqueous (Hardening), Non-Aqueous (Permanent)

Kit Contents:

  • 120 ml ImmPACT SG Diluent
  • 2.5 ml ImmPACT SG Reagent 1
  • 3 ml ImmPACT SG Reagent 2

Citations

Technical Information

Sections stained with the substrate also can be viewed by darkfield or electron microscopy. Vector SG and ImmPACT SG substrates can be used for both manual and automated staining methods. Stained sections can be dehydrated, cleared, and permanently mounted. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. 

SUBSTRATEsensitivity2 jpg

Substrate.CounterstainChart jpg SubstrateCombos jpg

Applicable patents and legal notices are available at legal notices.

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