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Maackia Amurensis Lectin I (MAL I), Fluorescein (FL-1311-2)

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Description

Maackia amurensis lectin I binds gal (β-1,4) glcNAc but tolerates substitution of N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.

Fluorescein labeled Maackia amurensis lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size2 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8°C
Maximum Excitation495-500 nm
Inhibiting and/or Eluting Sugar200 mM lactose (S-9004)
Maximum Emission514-521 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM CaCl2
Concentration2 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityGalactose, Lactose

Citations

Technical Information

This lectin is the leucoagglutinin or mitogenic isolectin from Maackia seeds.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011

Inhibiting Sugar: 200 mM lactose

Applicable patents and legal notices are available at legal notices.

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