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Ulex Europaeus Agglutinin I (UEA I), Biotinylated (B-1065-2)

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Description

Ulex europaeus agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.

Biotinylated Ulex europaeus agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageFor most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.08% sodium azide.
Concentration2 mg active conjugate/ml
ConjugateBiotinylated
Sugar SpecificityFucose, Arabinose

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 50 mM – 100 mM L-fucose

Applicable patents and legal notices are available at legal notices.

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