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Streptavidin, DyLight® 488 (SA-5488-1)

Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 488 labeled secondary antibody (green). S100, DyLight 594 labeled secondary antibody (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image c
Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image court
Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*
Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight 594 Goat Anti-Rabbit IgG (DI-1594); plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight 488 Streptavidin (SA-5488)
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight 594 Goat Anti-Rabbit IgG (DI-1594); plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight 488 Streptavidin (SA-5488)
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight 594 Goat Anti-Rabbit IgG (DI-1594); plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight 488 Streptavidin (SA-5488)
Streptavidin, DyLight™ 488
Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 488 labeled secondary antibody (green). S100, DyLight 594 labeled secondary antibody (red). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image c
Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image court
Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courte
Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*
Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight 594 Goat Anti-Rabbit IgG (DI-1594); plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight 488 Streptavidin (SA-5488)
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight 594 Goat Anti-Rabbit IgG (DI-1594); plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight 488 Streptavidin (SA-5488)
Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight 594 Goat Anti-Rabbit IgG (DI-1594); plaques stained with Biotinylated Mouse Anti-Human Amyloid Beta followed by DyLight 488 Streptavidin (SA-5488)
Streptavidin, DyLight™ 488
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Description

DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. 

Features:

  • Advantages of DyLight™ dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation maximum: 493 nm
  • Emission maximum: 518 nm
  • Color:  Green

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation493 nm
Maximum Emission518 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 488
Color of FluorescenceGreen
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. 

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

DyLightspectra jpg

Dylight Table1 jpg

Applicable patents and legal notices are available at legal notices.

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