Glycoproteins are isolated and purified from protein mixtures using columns of agarose-bound lectins. After the protein mixture is applied to the column, the column is washed free of unwanted proteins. Then, the glycoprotein is eluted with a sugar that inhibits binding (inhibiting sugar) or in some cases, with a special eluting solution.
Use Vector inhibiting sugars in glycoprotein research to:
Vector’s glycoprotein eluting solutions offer convenience and superior recovery over standard sugar solutions. They have a neutral pH, which maximizes the yield of eluted glycoproteins and preserves the activity of agarose-bound lectins for repeated use. All components of these ready-to-use elution solutions can be subsequently removed by dialysis.