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Blocking Reagents for Lectin Histochemistry

Clean, reliable, reproducible results can be attained by using appropriate detection reagents, running informative negative controls, and choosing the right blocking solutions. Negative controls will help determine the factors that contribute most to background staining. An appropriate series of controls is outlined in Vector's Trouble Shooting Guide (available for free download or as a convenient laminated copy for the lab).

Blocking agents are chosen based on results from these negative control sections, and the appropriate ones can prevent background interference from different sources including endogenous enzyme activity, presence of Fc receptors, or interactions of detection reagents with tissue/cell proteins and other macromolecules.

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