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Biotin Quantitation

Biotin Quantitation

The Quant*Tag™ Biotin Kit (BDK-2000) measures the amount of free biotin in solution or the number of biotins attached to nucleic acids, proteins, or other macromolecules. Use this kit to determine the labeling efficiency of biotin-labeled molecules.

Unlike conventional biotin quantitation methods like the HABA assay, the Quant*Tag™ Biotin Kit requires no predigestion of protein or nucleic acids, saving time and increasing accuracy. More sensitive than the HABA assay, the kit can detect less than 1 nmol of biotin. Quant*Tag™ Kit reagents chemically react with free or bound biotin producing a colored product that can be quantified using a spectrophotometer. The absorbance is measured in the visible spectrum allowing the use of plastic cuvettes or microtitre plates.

The Quant*Tag™ Biotin Kit is quick and easy to use, and the assay can be completed in 30 minutes. A biotin standard is included. The kit contains sufficient reagents to perform from 25 to 250 tests depending on assay size.

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  1. The sample to be tested is reacted with the kit reagents along with standards containing known amounts of biotin.  The absorbance readings of the known samples are plotted producing a standard curve.  The absorbance of the test sample is located on the st
    QuantTag Biotin Quantitation Kit
    Cat. No: BDK-2000
    Unit Size: 1 kit
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