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Peanut Agglutinin (PNA), Biotinylated

B-1075-5
SKU Unit Size Price Qty
B-1075-5 5 mg
$215.00
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Description

Peanut agglutinin binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. 

Biotinylated peanut agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

 

Specifications

More Information
Unit Size 5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage DO NOT VORTEX. For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer. Use of buffers containing 0.1 mMCaCl2 and 0.01 mM MnCl2 is recommended. Inhibiting/Eluting Sugar: 200 mM galactose (S-9003).
Recommended Storage 2-8 °C; Store frozen for long term storage
Inhibiting and/or Eluting Sugar Inhibiting/Eluting Sugar: 200 mM galactose (S-9003).
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2
Concentration 5 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity Galactose

Documents

Product FAQs

I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is meet. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Related Products

Galactose Galactose Catalog #: S-9003
Peanut Agglutinin (PNA), Rhodamine Peanut Agglutinin (PNA), Rhodamine Catalog #: RL-1072-5
Tonsil (FFPE): Antigen Retrieved with Antigen Unmasking Solution and stained with Cy3 PNA (red-orange). Mounted in VECTASHIELD HardSet with DAPI (blue). Peanut Agglutinin (PNA), CY3 Catalog #: CL-1073-1
Tonsil (FFPE): Antigen Retrieved with Antigen Unmasking Solution and stained with Cy5 PNA (purple). Stained with DAPI (blue). Peanut Agglutinin (PNA), CY5 Catalog #: CL-1075-1

Citations

Technical Information

PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding can be used to measure cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte subpopulations in man and experimental animals, and to measure the levels of lymphoid cell populations in many diseases. PNA can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.

A major cell surface receptor for PNA may be asialo GM1 ganglioside. Since PNA shares specificity with the antibody to this glycolipid, PNA and the antibody can be used interchangeably in some applications. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 200 mM galactose
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