These antibodies to lectins are produced by immunizing goats with pure lectins. Following conventional purification steps, specific antibody is isolated by affinity chromatography on lectin-agarose columns. Each anti-lectin is supplied lyophilized in buffered saline.
Biotinylated anti-lectins are optimally labeled with biotin.
10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose
Recommended Usage
The recommended concentration range for use is 5-20 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous
immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
These highly purified specific antibodies provide excellent intermediate reagents for localizing lectin receptors in tissues and on cells or following lectin transport in neuronal tracing.
Biotinylated anti-lectins can be used as intermediates to localize lectin receptors in tissues or on cells, or to follow lectin migration in neuronal transport. In some applications, biotinylated anti-lectins used in conjunction with unlabeled lectins may provide greater sensitivity than using biotinylated lectins alone.