The alkaline phosphatase substrate kits that form precipitates are based on either reduction of tetrazolium salts or the production of colored diazo compounds. Sections stained with the Vector® Red, BCIP/NBT, or Vector® Black substrate kits can be cleared with xylene. The Vector® Blue substrate product is soluble in xylene but can be cleared with citrus oil-based clearing agents and then permanently mounted. The Vector® Red and Vector® Blue substrate combination has been reported to be useful in co-localization of antigens. The BCIP/NBT substrate is frequently used for nitrocellulose or in situ hybridization applications.
The newest formulation, ImmPACT™ Vector® Red, provides the following advantages: