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dPEG®₄₈-biotin acid (QBD-10776)

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Description

dPEG®48 biotin acid, product number QBD-10776, is a biotinylation reagent containing a long (157 atoms), single molecular weight, discrete polyethylene glycol (dPEG®) spacer that is functionalized with biotin on one end and a propionic acid moiety on the other. The terminal propionic acid group couples to amines directly using a carbodiimide such as EDC, and it can be functionalized as an N-hydroxysuccinimidyl (NHS) or 2,3,5,6-tetrafluorophenyl (TFP) ester for reaction with amines.

Amphiphilic dPEG®48 biotin acid is soluble in water, aqueous buffers, and organic solvents. When used as a label, it does not cause aggregation or precipitation of biomolecules even when excess label is used. Using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry, QBD-10776 can be coupled directly to free primary amines in aqueous media. These free amines can be on a protein, peptide, or the treated surface of a nanoparticle. The linker length of dPEG®48 biotin acid is longer than the linker length of LC-biotin and longer than the linker length of LC-LC-biotin. The performance characteristics of dPEG®48-biotin acid are quite superior to both LC-biotin and LC-LC-biotin.

Many applications use this product to take advantage of the strong avidin-biotin binding interaction. Such applications include single-cell imaging of protein secretion, developing biosensors, isolating specific cell types by separation with magnetic beads, characterizing and understanding the streptavidin-biotin interaction, creating affinity-based probes, and assembling supramolecular nanostructures.

Specifications

Unit Size100mg, 1000mg
Molecular Weight2500.99; single compound
Chemical formulaC₁₁₂H₂₁₈N₄O₅₃S
CAS721431-18-1
Purity> 98%
SpacersdPEG® Spacer is 157 atoms and 187.8 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, DMSO or water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Surface Functionalization Using Catalyst-Free Azide-Alkyne Cycloaddition. Alexander Kuzmin, Andrei Poloukhtine, Margreet A. Wolfert, and Vladimir V. Popik. Bioconjugate Chem. 2010, 21 (11) pp 2076–2085. October 21, 2010. DOI: 10.1021/bc100306u.

Sensitive Detection of Small Molecule–Protein Interactions on a Metal – Insulator – Metal Label-Free Biosensing Platform Amir Syahir, Kotaro Kajikawa and Hisakazu Mihara. Chemistry Asian Journal. 2012, 8 (7), pp 1867-1874. August 2012. DOI: 10.1002/asia.201200138.

Prion protein 90-231 contains a streptavidin-binding motif. Thurid Boetel, Steffen Bade, Marcus Alexander Schmidt, Andreas Frey. Biochemical and Biophysical Research Communications. 2006, 349 (1) pp 296-302. October 13, 2006. DOI: 10.1016/j.bbrc.2006.08.041.

Macrocycles That Inhibit the Binding between Heat Shock Protein 90 and TPR-Containing Proteins. Veronica C. Ardi, Leslie D. Alexander, Victoria A. Johnson, and Shelli R. McAlpine. ACS Chem. Biol. 2011, 6 (12), pp 1357–1366. September 27, 2011. DOI: 10.1021/cb200203m.

Measurement of Inflammatory Cytokine Secretion from Human Monocytes after Inflammasome Activation. Yoshitaka Shirasaki, Asahi Nakahara, Nanako Shimura, Kazushi Izawa, Nobutake Suzuki, Mai Yamagishi, Jun Mizuno, Tetsushi Sekiguchi, Ryuta Nishikomori, Shuichi Shoji, Osamu Ohara. Royal Society of Chemistry. 2012, 978-0-9798064-5-2 pp 1012-1014. November 1, 2012. DOI: 12CBMS-0001.

Applicable patents and legal notices are available at legal notices.

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