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Difluorocarboxyfluorescein Azide, 6-isomer (FP-1225)

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Description

2′, 7′-Difluorocarboxyfluorescein Azide, 6-isomer (also under Oregon Green 488 Azide (Oregon Green™ 6-Carboxamido-(6-Azidohexanyl), Triethylammonium Salt), 6-isomer name) is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

2′,7′-Difluorocarboxyfluorescein fluorinated analog of fluorescein that overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityAlkynes, cyclooctynes
Abs/Em Maxima496/524 nm
Extinction coefficient78,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesAlexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight637.68
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

MB488

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