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DBCO-PEG5-NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.


Unit Size4x2mg, 20mg, 25mg, 100mg, 500mg, 1g
Molecular weight693.74
Chemical compositionC36H43N3O11
SolubilityDMSO, DMF, DCM, THF, Chloroform
Purity>95% (HPLC)
AppearanceViscous oil or amorphous solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Selected References

  1. Tracy, C. M., et al. (2019). Cysteine-Directed Bioconjugation of a Platinum(II)-Acridine Anticancer Agent. Inorg Chem.58 (1), 43-46. [PubMed]
  2. Allo, B., et al. (2018). Clickable and High-Sensitivity Metal-Containing Tags for Mass Cytometry. Bioconjugate Chem. 29(6), 2028-38. [PubMed]
  3. Nedrow-Byerst, J.R., et al. (2013). PSMA-targeted SPECT agents: mode of binding effect on in vitro performance. Prostate.73(4), 355-62-9. [PubMed]

Applicable patents and legal notices are available at legal notices.

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