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Cy5 Azide (CCT-AZ118)

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This product was previously sold as 1324 from Fluoroprobes.

Description

The far-red fluorescent Cy5 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This far-red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 633 nm or 647 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
Abs/Em Maxima649/671 nm
Extinction Coefficient
250,000
Flow Cytometry Laser Line
633 or 635 nm
Microscopy Laser Line
633 or 635 nm
Spectrally Similar DyesAlexa Fluor® 647, CF™ 647 Dye, DyLight™649
Molecular weight834.01 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceBlue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy Cy

Selected References

  1. Heybrock, S., et al. (2021). S-palmitoylation determines TMEM55B-dependent positioning of lysosomes. J Cell Sci.135 (5), jcs258566. [PubMed]

Applicable patents and legal notices are available at legal notices.

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