The far-red fluorescent Cy5 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This far-red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 633 nm or 647 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.
| Unit Size | 1 mg, 5 mg, 25 mg, 100 mg |
|---|---|
| Abs/Em Maxima | 649/671 nm |
| Extinction Coefficient | 250,000 |
| Flow Cytometry Laser Line | 633 or 635 nm |
| Microscopy Laser Line | 633 or 635 nm |
| Spectrally Similar Dyes | Alexa Fluor® 647, CF™ 647 Dye, DyLight™649 |
| Molecular weight | 834.01 (protonated) |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Blue solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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