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Cy5.5 DBCO (CCT-1046)

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This product was previously sold as 1347 from Fluoroprobes.

Cy5.5 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures allowing detection of azide-tagged biomolecules under mild conditions. In application where the presence of copper is a concern Cy5.5 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Description

Cy5.5 DBCO is a bright and photostable near-IR probe that spectrally similar to Alexa Fluor® 680, DyLight® 680, and IRDye® 680 dye. The Cy5.5 DBCO is water-soluble, hydrophilic dye often a reagent of choice for assay where minimal non-specific binding and exceptional brightness is required. The fluorescence of Cy5.5 DBCO is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.

Cy5.5 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy5.5 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Cy5.5 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
Abs/Em Maxima678/694 nm
Extinction Coefficient
190,000
Spectrally Similar DyesAlexa Fluor® 680, IRDye® 680RD, DyLight® 680
Molecular weight1161.34
CAS1857352-95-4
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceBlue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy5.5 Cy5.5

Selected References

  1. Lancien, M., et al. (2020). A snake toxin as a theranostic agent for the type 2 vasopressin receptor. Theranostics.10 (25), 11580-11594. [PubMed]
  2. Wang X., et al. (2021). Equipping Natural Killer Cells with Cetuximab through Metabolic Glycoengineering and Bioorthogonal Reaction for Targeted Treatment of KRAS Mutant Colorectal Cancer. ACS Chem. Biol., 16 (4), 724-730. [PubMed]

Applicable patents and legal notices are available at legal notices.

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