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One-Step Detection

One-Step Fluorescence Detection. Fluorochrome conjugated secondary antibodies may be used to detect an unconjugated primary antibody on a blot. These fluorescent detection reagents are used in conjunction with the proper membrane and visualization equipment. This method is fast and simple and allows multiple targets to be visualized simultaneously.

One-Step Enzymatic Detection. One-step enzymatic detection offers greater sensitivity and more versatility due to the types of detection reagents and enzyme substrates available. For one-step detection, alkaline phosphatase (AP) and peroxidase (HRP) conjugated secondary antibodies are available.

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